中国药业2016，Vol.25Issue(9)：8-11,12,5.
盾叶薯蓣中薯蓣总皂苷生物酶预处理法提取工艺研究
Extraction Process of Enzyme Pretreatment Method for Extracting Total Saponins from Dioscorea
摘要
Abstract
Objective To establish a highly efficient extraction method for the saponins extracted from dioscorea zingiberensis. Methods Evaluated by the yield of the saponins, the 5 factors, namely temperature, pH, water consumption, dosage of enzyme and enzyme hydrolysis time in the process of enzyme pretreatment were optimized via single factor experiment, and the 4 factors, namely ethanol concentration, ratio of solid to liquid, extracted time and extraction degree in the saponins extracion process were optimized via orthogonal exper-iment. Results The best conditions in the enzyme pretreatment are as follows:the temperature for the enzyme was 70 ℃, pH 5. 5, water consumption was 4 L/kg, dosage of enzyme was 8 mL/kg, enzyme hydrolysis time was 24 h;the best conditions in the saponins ex-tracted process were as follows: ethanol concentration 60% , ratio of solid to liquid 1: 8, extraction time 1. 0 h, extraction degree twice. Conclusion The optimal conditions can extract the saponins from dioscorea zingiberensis stably.关键词
盾叶薯蓣/生物酶预处理/薯蓣总皂苷/提取工艺Key words
dioscorea/enzyme pretreatment/total saponins/extraction process分类
医药卫生引用本文复制引用
杨光义,雷攀,杜士明,惠小娜,叶方,张晨宁,魏晋宝..盾叶薯蓣中薯蓣总皂苷生物酶预处理法提取工艺研究[J].中国药业,2016,25(9):8-11,12,5.基金项目
2013年湖北省科技支撑计划（对外科技合作类）项目，项目编号2013BHE017；2014年湖北省教育厅中青年创新团队项目，项目编号T201414。 （）
