中国药业2016，Vol.25Issue(9)：65-67,3.
反相高效液相色谱法同时测定香菊胶囊中绿原酸和咖啡酸含量
Simultaneous Content Determination of Chlorogenic Acid and Caffeic Acid in Xiangju Capsules by RP-HPLC
孙菲 1孙欣 1金荣1
作者信息
- 1. 黑龙江省鸡西市食品药品检验检测中心，黑龙江 鸡西 158100
- 折叠
摘要
Abstract
Objective To establish an RP-HPLC method for simultaneous content determination of chlorogenic acid and caffeic acid in Xiangju Capsules. Methods The Agilent Zobax SB-C18 colume ( 250 mm × 4. 6 mm, 5 μm ) was used; the mobile phase was acetoni-trile-methanol-0. 2%H3PO4 by gradient elution and the detection wavelength was 328 nm;the flow rate was 1. 0 mL/min, the injec-tion volume was 20 μL, and the column temperature was 30 ℃. Results The linear response of chlorogenic acid ranged from 2. 034 × 10-2-5. 085 × 10-1μg ( r=0. 999 9,n=7 ) , and the linear response of caffeic acid ranged from 2. 218 × 10-2-5. 545 × 10-1μg ( r=0. 999 9,n=7 );the average recovery rate of chlorogenic acid was 98. 83% ( RSD=0. 86%,n=6 ) , and the average recovery of caffeic acid was 98. 72% ( RSD=0. 88%,n=6 ) . Conclusion The method is convenient, quick and accurate, and can be used for the quality control of Xiangju Capsules.关键词
香菊胶囊/含量测定/绿原酸/咖啡酸/高效液相色谱法Key words
Xiangju Capsules/content determination/chlorogenic acid/caffeic acid/HPLC分类
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孙菲,孙欣,金荣..反相高效液相色谱法同时测定香菊胶囊中绿原酸和咖啡酸含量[J].中国药业,2016,25(9):65-67,3.
