中国中医药信息杂志2016，Vol.23Issue(6)：56-59,4.DOI:10.3969/j.issn.1005-5304.2016.06.015
育肾助孕方对雷公藤多苷致卵巢储备下降大鼠卵巢形态和性激素的影响
Effects ofYushen Zhuyun Prescription on Ovarian Morphology and Sex Hormone of Rats with Decreased Ovarian Reservation Induced by Tripterygium Glycosides
摘要
Abstract
ObjectiveTo observe the effects ofYushen Zhuyun Prescription on ovarian morphology and sex hormone of rats with decreased ovarian reservation (DOR) induced by tripterygium glycosides; To explore its mechanism of action.Methods Tripterygium glycosides solution was used to establish DOR model rats by gastric gavage. 48 female SD rats were randomly divided into blank group, model group, positive medicine group andYushen Zhuyun Prescription low-, medium- and high-dose groups.Yushen ZhuyunPrescription groups were given the low, medium, high dosagesYushen Zhuyun Prescription liquid; positive medicine group were given estradiol valerate liquid; model group and blank control group were given normal saline with the same amount, by gavage 2 times per day for 14 consecutive days. The general condition of the rats was observed; ovarian was weighed and the viscera index was calculated; ovarian morphology was observed by HE staining; the levels of serum estradiol (E2), follicle stimulating hormone (FSH) and testosterone (T) were detected by radioimmunoassay; the levels of inhibin B (INHB) and anti Mullerian hormone (AMH) were detected by ELISA.Results Compared with the blank group, ovarian tissue atrophied; the number of follicles was reduced; ovarian index and the level of E2, INHB and AMH decreased; the levels of FSH and LH increased in the model group (P<0.01). Compared with the model group, ovarian tissue morphology improved significantly; the number of follicle and corpus luteum increased; follicular atresia was reduced; ovarian index and the levels of E2, INHB, and AMH increased; the levels of FSH and LH decreased in Yushen Zhuyun Prescription high-dose group (P<0.01). INHB and AMH had significant correlation (P<0.01). ConclusionYushen Zhuyun Prescription can regulate hormone levels, promote the growth and secretion of follicle, and inhibit follicular atresia, thereby improve ovarian reserve function.关键词
育肾助孕方/卵巢储备功能/抑制素B/抗苗勒管激素/大鼠Key words
Yushen Zhuyun Prescription/ovarian reservation/inhibin B/anti Mullerian hormone/rats分类
医药卫生引用本文复制引用
金毓莉,张婷婷,许华云,袁雪菲,蔡小荪..育肾助孕方对雷公藤多苷致卵巢储备下降大鼠卵巢形态和性激素的影响[J].中国中医药信息杂志,2016,23(6):56-59,4.基金项目
国家中医药管理局中医学术流派传承工作室（2013年）；国家中医药管理局重点专科资助项目（ZK0901FK012）；上海市进一步加快中医药事业发展三年行动计划 （）
