中国中医药信息杂志2016，Vol.23Issue(6)：73-76,4.DOI:10.3969/j.issn.1005-5304.2016.06.019
解毒化瘀方药物血清与药物血浆对模型PC12细胞蛋白酶活化受体-1、细胞外信号调节激酶1/2表达的影响
Effects ofJiedu HuayuPrescription Containing Serum and Plasma on PAR-1 and ERK1/2 of PC12 Cell Model
摘要
Abstract
Objective To compare the differences between Jiedu HuayuPrescription containing serum and plasma on PAR-1, ERK1/2 expression of the PC12 cell injury model.Methods Rat serum containing and plasma models was prepared through gavage. Three gas incubators and sugar-free DMEM medium simulated ischemia caused by hypoxia were used, and 150 U/mL of thrombin was added, with a purpose to establish the thrombin-induced hypoxic PC12 cell injury model. The experiment was divided as control group, model group, serum containing group and plasma containing group. Real-time fluorescent quantitative PCR was used to detect the expression levels of PAR-1 and ERK1/2 mRNA in each group; immunofluorescence was used to detect the protein expressions of PAR-1 and ERK1/2. Results Compared with the control group, the expressions of PAR-1 and ERK1/2 mRNA and protein in model group were enhanced (P<0.05); compared with the model group, the expressions of PAR-1 and ERK1/2 mRNA and protein in serum containing group and plasma containing group decreased (P<0.05,P<0.01); the expressions of PAR-1 and ERK1/2 mRNA and protein in plasma containing group decreased more significantly than serum containing group (P<0.05).ConclusionJiedu Huayu Prescription containing plasma was superior to its containing serum against the injury of PC12 cell induced by hypoxic and thrombin.关键词
解毒化瘀方/药物血浆/药物血清/PC12细胞/大鼠Key words
Jiedu HuayuPrescription/plasma containing medicine/serum containing medicine/PC12 cells/rats分类
医药卫生引用本文复制引用
邓奕辉,李银,谭琥,覃弘宇,李定祥..解毒化瘀方药物血清与药物血浆对模型PC12细胞蛋白酶活化受体-1、细胞外信号调节激酶1/2表达的影响[J].中国中医药信息杂志,2016,23(6):73-76,4.基金项目
国家自然科学基金（81373508）；湖南省自然科学基金 （）
湖南省中医药科研基金资助项目（201319）；湖南省教育厅资助项目（12A105）；湖南省重点学科中西医结合基础资助项目（2011-2015）；湖南省自然科学创新群体基金（2012年）；湖南省高校科技创新团队 （）
