作物学报2016，Vol.42Issue(6)：787-794,8.DOI:10.3724/SP.J.1006.2016.00787
9311与日本晴间一个杂种不育基因的鉴定与定位
Identification and Mapping of a Hybrid Sterility Gene between 9311 and Nip-ponbare
摘要
Abstract
Exploitation of subspecific heterosis is an effective method to improve rice yield by overcoming hybrid sterility between subspecies. In this study, F1 plants of the cross between Nipponbare and T9424, a line from a set of chromosome segment substitution lines with Nipponbare background as recipient and 9311 as donor, showed the decreasing spikelet and pollen fertility compared with the two parents, indicating that there was the incompatibility between the parents. Three substituted chromosome segments on chromosome 1, 4, and 5, respectively, were identified by whole genome re-sequencing of T9424. Analysis of the genotypes and spikelet fertility of plants in Nipponbare/T9424 F2 population indicated that hybrid sterility gene between T9424 and Nipponbare was located on chromosome 5. A total of 790 plants were then used for mapping the hybrid sterility gene, and the target gene was mapped to a candidate region with the physical distance of 110 kb between PSM8 and A14 on chromosome 5. The hybrid sterility gene, named S39(t) temporarily, controlled partial abortion of both pollen grains and embryo-sac of Nippon-bare/T9424 F1 plants. These results are useful for deepening understanding of the phenomenon of hybrid sterility, and lay the groundwork for the gene cloning and its use in breeding.关键词
水稻/杂种不育基因/基因定位Key words
Rice/Hybrid sterility gene/Gene mapping引用本文复制引用
张宏根,张丽佳,孙一标,司华,刘巧泉,汤述翥,顾铭洪..9311与日本晴间一个杂种不育基因的鉴定与定位[J].作物学报,2016,42(6):787-794,8.基金项目
本研究由国家重点基础研究发展计划项目(2011CB100101)和江苏高校优势学科建设工程项目资助。This study was supported by the Key Project of Chinese National Programs for Fundamental Research and Development (2011CB100101) and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions （2011CB100101）
