作物学报2016，Vol.42Issue(6)：795-802,8.DOI:10.3724/SP.J.1006.2016.00795
小麦全基因组NBS类R基因分析及2AL染色体NBS-SSR特异标记开发
Genome-Wide Analysis of TaNBS Resistance Genes and Development of Chromosome 2AL-specific NBS-SSR Markers in Wheat
摘要
Abstract
Nucleotide binding site (NBS)-encoding genes are the most important resistance genes (R genes) in plant kingdom. In this study, 2406 full-length NBS protein sequences, of which single protein varies from 48 aa to 2272 aa, were identified from wheat (Triticum aestivum L.) genome using bioinformatic method. These TaNBSs were divided into four categories, including N, CN, NL, and CNL, according to whether the NBS domains connect CC or LRR domains at both ends. Diagnosis results showed that 1203 of all the scaffolds with TaNBS contained 2177 simple sequence repeats (SSR) loci, 73.5%of which were dinucleotide repeat sites. Based on the NBS-SSR loci on chromosome 2AL in wheat, we developed 51 molecular markers, and 39 of them (76.5%) were confirmed as chromosome specific markers using Chinese Spring nulli-tetrasomic and ditelosomic lines. Further-more, 24 2AL-specific NBS-SSR markers showed polymorphism between resistant material Khapli carried Pm4a on chromosome 2AL and susceptible material Chancellor. Finally, three 2AL-specific NBS-SSR markers, Sxaas_2AL22, Sxaas_2AL39, and Sxaas_2AL46, were probably linked to Pm4a gene based on genetic linkage test using Pm4a-NILs (Khapli/8*Cc). These chro-mosome specific 2AL-NBS-SSR markers can be used to locate novel R genes or screen the candidate sequences for known R genes on chromosome 2AL.关键词
小麦/抗病/NBS类基因/SSR标记/2AL染色体Key words
Wheat/Disease resistance/NBS-encoding genes/SSR markers/Chromosome 2AL引用本文复制引用
乔麟轶,常建忠,郭慧娟,高建刚,郑军,畅志坚..小麦全基因组NBS类R基因分析及2AL染色体NBS-SSR特异标记开发[J].作物学报,2016,42(6):795-802,8.基金项目
本研究由国家自然科学基金项目(31171839,31401385),山西省青年基金项目(2015021145),山西省农业攻关项目(20150311001-1,20150311001-5),山西省农业科学院攻关项目(15YGG01)和北京市农林科学院青年基金项目(QNJJ201428)资助。This study was supported by the National Natural Science Foundation of China (31171839,31401385), Shanxi Province Science Foundation for Youths (2015021145), Shanxi Province Technologies R&D Program (20150311001-1,20150311001-5), the Technologies R&D Program of the Shanxi Academy of Agricultural Sciences (15YGG01), and the Youth Foundation of Beijing Academy of Agricultural and Forestry Sciences (QNJJ201428) （31171839,31401385）
