作物学报2016，Vol.42Issue(6)：813-819,7.DOI:10.3724/SP.J.1006.2016.00813
水稻颖壳退化突变体degenerated hull 3（dh3）的表型分析与基因定位
Phenotypic Analysis and Gene Mapping of degenerated hull 3 (dh3) Mutant in Rice (Oryza sativa L.)
摘要
Abstract
The floral organ development directly influences the yield and quality of rice. In this study, we reported a mutant de-generated hull 3 (dh3), derived from ethylmethane sulfonate (EMS)-treated Jinhui 10 (Oryza sativa L. ssp. indica). The manifes-tation of dh3 mutant was degradation and narrowing in lemma and palea, losting the ability of close-up. In some seriously mutated florets, the lemma even degenerated in an awn-like shape, the margin region of palea and lodicules became narrow, even fused. Genetic analysis indicated that the dh3 character is controlled by a recessive gene. By using 356 F2 mutants derived from a cross between sterile line Xinong 1A and dh3, DH3 was mapped between SSR markers RM27706 and RM27709 on chromosome 12 in rice, with a physical distance of 44.72 kb. There is no report of known functional gene in this zone. The results will shed light on the future clone and function analysis of DH3 gene.关键词
水稻/颖壳退化/表型分析/基因定位Key words
Rice (Oryza sativa)/degenerated hull/Phenotypic analysis/Gene mapping引用本文复制引用
龙珏臣,李云峰,庄慧,陈欢,汪玲,沈亚林,曾晓琴,崔馨允,桑贤春,何光华..水稻颖壳退化突变体degenerated hull 3（dh3）的表型分析与基因定位[J].作物学报,2016,42(6):813-819,7.基金项目
本研究由国家自然科学基金项目(31271304),重庆市自然科学重点项目(cstc2012jjB80005)和中央高校基本科研业务费(XDJK2012A001)资助。This work was supported by the National Natural Science Foundation of China (31271304), the Natural Science Foundation Project of Chongqing (CSTC2012JJB80005), and the Fundamental Research Funds for the Central Universities (XDJK2012A001) （31271304）
