作物学报2016，Vol.42Issue(6)：832-843,12.DOI:10.3724/SP.J.1006.2016.00832
亚洲棉bZIP蛋白家族的鉴定及GaFD基因的组织表达分析
Identification of bZIP Protein Family in Gossypium arboreum and Tissue Ex-pression Analysis of GaFDs Genes
摘要
Abstract
The basic leucine zipper (bZIP) is one of the largest and most diverse transcription factors in eukaryotes, and is in-volved in various processes of plant growth and development and in response to biotic and abiotic stresses. In this study, 159 bZIP family genes were identified and their complete gene sequences were obtained by using bioinformatics analysis method, based on Gossypium arboreum whole genome database, and 159 bZIP genes were loaded on 13 chromosomes. The 159 bZIP genes were categorized into 13 groups based on their phylogenetic relationships, gene structures and conserved motifs. In addition, three GaFD homologous genes GaFD1, GaFD2, and GaFD3 were identified, which belongs to A subfamily in G. arboretum. The ex-pression patterns of GaFD genes in different tissues were determined by using quantitative Real-time reverse transcription PCR (qRT-PCR) method. The results showed that GaFD1 and GhFD2 were preferentially expressed in the shoot apical meristem (SAM), whereas GaFD3 was preferentially expressed in stem. The results revealed that a number of bZIP family members exist in cotton genome, and FD genes with different structures and expression patterns play different roles in the development of cotton, which provides valuable information for dissecting the function and mechanism of bZIPs in cotton.关键词
碱性亮氨酸拉链/亚洲棉/FD/进化分析/基因表达Key words
Basic leucine zipper/Gossypium arboreum/FD/Phylogenetic analysis/Gene expression引用本文复制引用
张彦楠,蔡大润,黄先忠..亚洲棉bZIP蛋白家族的鉴定及GaFD基因的组织表达分析[J].作物学报,2016,42(6):832-843,12.基金项目
本研究由教育部新世纪优秀人才支持计划项目(NCET-12-1072),国家自然科学基金项目(31360366)和新疆生产建设兵团博士基金项目(2012BB007)资助。This study was supported by the Program for New Century Excellent Talents in University of Ministry of Education of China (NCET-12-1072), the National Natural Science Foundation of China (31360366), and Program for Doctor Foundation in XinJiang Production and Construction Corps (2012BB007) （NCET-12-1072）
