作物学报2016，Vol.42Issue(6)：860-872,13.DOI:10.3724/SP.J.1006.2016.00860
小麦籽粒游离多胺对土壤干旱的响应及其与籽粒灌浆的关系
Free Polyamines in Grains in Response to Soil Drought and Their Relationship with Grain Filling of Wheat
摘要
Abstract
For understanding the role of endogenous free polyamines on grain filling of wheat under drought stress, we conducted a two-year pot experiment from September 2013 to June 2015 using high-yield wheat cultivars Yangmai 16 and Ningmai 13 grown in different soil moisture conditions. Three treatments, namely well-watered (WW), moderate soil-drought (MD), and se-vere soil-drought (SD), were imposed from late-tillering to maturity stage. Grain filling rate and free polyamines levels in both superior and inferior grains were determined. The results showed the consistency between the two cultivars. Compared with WW, MD treatment had significantly increased grain-filling rate and grain weight in inferior grains by 12.5%and 11.8%, respectively;whereas no effect on grain filling in superior grains. In contrast, SD treatment showed negative influences on leaf water potential, photosynthetic rate, and grain filling. Under SD treatment, grain-filling rate and grain weight of superior grains reduced by 10.1%and 9.5% and those of inferior grains reduced by 14.5% and 11.7%, respectively. During grain filling, concentrations of free spermidine (Spd) and spermine (Spm) as well as their ratios to putrescine (Put) in inferior grains increased significantly under MD treatment and decreased significantly under SD treatment. Grain-filling rate and grain weight were positively correlated with con-centrations of Spd and Spm, and the ratios of Spd/Put and Spm/Put (P<0.01), whereas negatively correlated with Put concentra-tion (P < 0.01). Exogenous Spd or Spm resulted in significant increases of grain-filling rate (11.2–25.9%) and grain weight (9.9–17.7%) in inferior grains under the three soil moistures and in superior grains under SD treatment, and had no significant difference in superior grains between WW and MD treatments. The positive effects of exogenous Spd and Spm were eliminated when their synthesis inhibitor, methylglyoxal-bis guanylhydrazone (MGBG), was applied together with Spd and Spm. Both supe-rior and inferior grains showed great decreases of grain-filling rate (20.5–28.8%) and grain weight (16.9–28.5%) after spraying MGBG under the three soil moistures. These results indicate that the responses of polyamines in grain to soil moisture vary with drought strength, and moderate drought stress has a positive effect on grain filling through increasing concentrations of Spd and Spm and the ratios of Spd/Put and Spm/Put in grains.关键词
小麦/土壤干旱/多胺/籽粒灌浆/粒重Key words
Wheat/Soil drought/Polyamines/Grain filling/Grain weight引用本文复制引用
张伟杨,徐云姬,钱希旸,李银银,王志琴,杨建昌..小麦籽粒游离多胺对土壤干旱的响应及其与籽粒灌浆的关系[J].作物学报,2016,42(6):860-872,13.基金项目
本研究由国家自然科学基金项目(31271641,31471438),中央级科研院所基本科研业务费(农业)专项(201103003,201203079),国家科技支撑计划项目(2011BAD16B14,2012BAD04B08,2013BAD07B09,2014AA10AS605),江苏省农业三新工程项目(SXG2014313)和江苏高校优势学科建设工程专项资助。This study was supported by the National Natural Science Foundation of China (31271641,31471438), China National Public Welfare Indus-try (Agriculture) Plan (201103003,201203079), the National Key Technologies R&D Program of China (2011BAD16B14,2012BAD04B08,2013BAD07B09,2014AA10AS605), Jiangsu “Three-innovation” Agricultural Project (SXG2014313), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) （31271641,31471438）
