中国现代中药2016，Vol.18Issue(5)：547-553,572,8.DOI:10.13313/j.issn.1673-4890.2016.5.002
基于 UPLC-Q-TOF-MS 技术分析石吊兰全草化学成分
Study on Chemical Constituents in Whole Herb of Lysinotus pauciflorus by UPLC-Q-TOF-MS
摘要
Abstract
Objective:To analyze the chemical components in the whole herb of Lysinotus.pauciflorus Maxim by Ultra high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS).Methods:Crude drug were extracted with 80% methanol in water.A Waters ACQUITY UPLC-BEH-C18 S column (2.1 mm ×100 mm, 1.7 μm)was used with a gradient elution of acetonitrile-water containing 0.1% formic acid.The mass spectrometry equipped with ionization source was used and the data was collected under negative ion mode.And the components identification was applied by the precise molecular weight and fragmentation information,combined with a database match.Results:Forty-eight compounds were identified with 42 phenylethanoid glycosides,3 flavonoids and 3 other compounds,among them,14 compounds were firstly identified in Gensneriaceae plant.Conclusion:We have quite completely identified the components in the whole herb of L.pauciflorus for the first time,which lay the foundation for the further study and utilization of the medicinal resources.关键词
液质联用/石吊兰/化学成分Key words
UPLC-Q-TOF-MS/Lysinotus pauciflorus Maxim./chemical constituents引用本文复制引用
胡峻,刘勇,齐梦蝶,张权,秦振娴,康利平,南铁贵,杨健,袁媛,詹志来..基于 UPLC-Q-TOF-MS 技术分析石吊兰全草化学成分[J].中国现代中药,2016,18(5):547-553,572,8.基金项目
［基金项目］中医药行业科研专项 （）
