中国现代中药2016，Vol.18Issue(5)：573-578,6.DOI:10.13313/j.issn.1673-4890.2016.5.007
儿茶酚香豆素清除自由基能力测定及作用机制探索
Free Radical Scavenging Capability of Catechol Coumarins and Mechanism
摘要
Abstract
Objective:To evaluate free radical scavenging capacity of catechol coumarin,and further explore the mechanism of the reaction between catechol coumarins and free radical.Methods:DPPH,ABTS·+ scavenging activity of a series of coumarins including substituent group at position 4,8 and monomethylation products were assayed,then the curve of logc-scvenging activity was drawn.Finally,the free radical scavenging mechanism of catechol compounds was explored according to the literature.Results:All of compounds investigated possessed excellent antioxidant activity,and the ability to get rid of ABTS·+ was stronger than that of DPPH.The ability of getting rid of DPPH was reduced by introducing nitrogen-containning groups,which acting as hydrogen bond acceptors for formation of intramolecular hydrogen bonds,while no significant changes in ABTS·+were found.However,the antioxidant activity decreased significantly,even to inactivation,for monomethylation of catechol coumarins.Conclusion:The mechanism complies with Hydrogen Atom Transfer (HAT)for scavenging DPPH,while sequential proton loss electron transfer may be more appropriate for ABTS·+.关键词
儿茶酚香豆素/自由基/作用机制Key words
Catechol coumarins/free radical/mechanism引用本文复制引用
陆俊霞,李珂珂,王平,崔攀,陈丽荣,弓晓杰..儿茶酚香豆素清除自由基能力测定及作用机制探索[J].中国现代中药,2016,18(5):573-578,6.基金项目
［基金项目］国家自然科学基金（81172949）；辽宁省优秀人才支持计划（LR2013058）；辽宁省自然科学基金计划（2015020657）；辽宁省科技厅科学技术计划（2014204007）；大连市科技局科技计划 （）
