中国现代中药2016，Vol.18Issue(5)：616-619,4.DOI:10.13313/j.issn.1673-4890.2016.5.017
广西莪术叶斑病病原鉴定及生物学特性研究
Identification of Curcuma kwangsiensis Leaf Spot Pathogen and Observation of Its Biological Characteristics
摘要
Abstract
Objective:The study is aimed to iendtify the organism that leads to the Curcuma kwangsiensis leaf spot andgrasp the biological characteristics of the pathogen.Methods:The fungus was isolated from infected plants according to Koch’s Rule.The identification of the pathogen was carried out mainly based on the morphological characters and molecular analysis of internal transcribed spacer(ITS)sequences;The effects of temperature,humidity,PH and nutrition on mycelial growth and conidialgermination of the pathogen were also investigated.Results:The morphological characters of the pathogenic isolate were in agreement with Phomopsis sp.When compared with sequences in GenBank,the ITS sequence of the pathogen was 99% similar to that of P.longicolla.On the condition of PH5 ~6,20 ~30 ℃ the fungal isolates grow bette and the more conidiums germinate.PDA medium with rhizomazedoariae juice was the optimum medium.Conclusion:The pathogen causing C.kwangsiensis leaf spot was identifidied as P.longicolla.The fungal isolates grow better on the condition of medium temperature,humidity and weak acid environment.关键词
广西莪术/病害/病原鉴定/生物学特性Key words
Curcuma kwangsiensis/disease/pathogen identification/biological characteristic引用本文复制引用
蒋妮,刘丽辉,胡凤云,刘威,刘凡..广西莪术叶斑病病原鉴定及生物学特性研究[J].中国现代中药,2016,18(5):616-619,4.基金项目
［基金项目］广西中医药科技专项（GZKZ1133）；南宁市科技成果推广及产业化示范项目 （）
