重庆医学Issue(22)：2571-2573,3.DOI:10.3969/j.issn.1671-8348.2013.22.002
SphK1-S1P信号通路的激活参与糖尿病大鼠肾损伤的研究
Activation of SphK1-S1P signaling pathway is involved in diabetic rat renal injury
摘要
Abstract
Objective We aimed to investigate whether SphK1-S1P signaling pathway is activated in diabetic nephropathy.Meth-ods SphK1 activity and S1P levels were measured by LC-MS/MS analysis.The expression of SphK1 were examined by immuno-histochemistry,real-time PCR and Western blot.Results Fasting blood glucose,kidney/body weight ratio and 24-h albuminuria were significantly increased in diabetic mice.Furthermore,mesangial hypertrophy was found in diabetic kidney.Strikingly,the stai-ning,activity and levels of mRNA and protein of SphK1,and S1P production in diabetic kidney were also markedly increased in dia-betic mouse kidney.Conclusion These results demonstrate that the SphK1-S1P signaling pathway is activated in diabetic kidney, suggesting that SphK1-S1P signaling pathway is implicated in the pathogenesis of diabetic nephropathy.关键词
SphK1-S1P信号通路/纤维连接蛋白/糖尿病肾病/STZKey words
SphK1-S1P signaling pathway/fibronectin/diabetic nephropathy/STZ引用本文复制引用
兰天,常秀亭,勾红菊,吴腾,王丽京,黄河清..SphK1-S1P信号通路的激活参与糖尿病大鼠肾损伤的研究[J].重庆医学,2013,(22):2571-2573,3.基金项目
国家自然科学基金资助项目（81170676；81200308；31271455）。 （）
