重庆医学Issue(22)：2611-2612,2615,3.DOI:10.3969/j.issn.1671-8348.2013.22.016
ELISA检测唾液中乙型肝炎表面抗原方法的优化与评价
Evaluation of modified ELISA method to detect Hepatitis B surface antigen in saliva specimens
摘要
Abstract
Objective To evaluated a modified ELISA method for detecting the Hepatitis B surface antigen(HBsAg)in saliva samples.Methods Optimized the different conditions,including sample type,sample volume and incubation condition,to screen the best condition.Three different methods to calculate cut-off value was evaluated to screen the most acceptable.Results The non-processed saliva sample can be used directly for detection(P=0.100).Test results of different sample volume was no significant difference(P=0.070).HBsAg was detected in 44 saliva samples out of 47 paired positive serum specimens and not detected in 63 saliva samples out of 68 matched negative serum samples by the optimized ELISA assay.There was excellent agreement between the results for the serum and saliva specimens and the kappa value was 0.87 for saliva specimens.Using an optimized protocol,the sensitivities and specificities were 93.6% and 92.6%,respectively.Conclusion Our data showed a significant promise for the use of the modified commercial ELISA in saliva sample for Hepatitis B virus infection surveillance.关键词
乙型肝炎病毒表面抗原/唾液/ELISAKey words
HBsAg/saliva/ELISA引用本文复制引用
张进..ELISA检测唾液中乙型肝炎表面抗原方法的优化与评价[J].重庆医学,2013,(22):2611-2612,2615,3.
