南方医科大学学报Issue(9)：1285-1288,4.DOI:10.3969/j.issn.1673-4254.2013.09.06
人凋亡诱导因子C-末端截短体AIFΔ1-480的表达对MCF-7细胞的影响
Effect of the C-terminal truncated human apoptosis-inducing factorΔ1-480 on biological behaviors of MCF-7 cells
摘要
Abstract
Objective To observe the expression of the C-terminal truncated human apoptosis-inducing factor (AIF) and its biological effect on MCF-7 cells. Methods PcDNA3.0-FDT-AIFΔ1-480 was transfected into human breast carcinoma MCF-7 cells with lipofectamins. The expression of the truncated AIF gene was detected by Western blotting, and its effects on the biological behaviors of MCF-7 cells and on the expression of cytochrome c (cytC) were evaluated using flow cytometry, MTT assay, colony-forming assay, and mitochondrial membrane potential measurement. Results PcDNA3.0-FDT-AIFΔ1-480 enhanced AIF expression in MCF-7 cells, obviously inhibited the cell proliferation, and significantly reduced the mitochondrial membrane potentials (P<0.05). Transfection of the cells with PcDNA3.0-FDT-AIFΔ1-480 promoted the expression of cytC and resulted in significantly increased apoptosis of MCF-7 cells (P<0.05). Conclusion The expression of C-terminal truncated human AIF gene can induce apoptosis of human MCF-7 cells by promoting cytC release from mitochondria.关键词
AIFΔ1-480/MCF-7细胞/增殖/凋亡/细胞色素CKey words
C-terminal truncated human apoptosis-inducing factorΔ1-480/MCF-7 cells/proliferation/apoptosis/cytochrome c引用本文复制引用
王丽娟,肖华卫,白娥,周霞,李萌,金桂花,朱青..人凋亡诱导因子C-末端截短体AIFΔ1-480的表达对MCF-7细胞的影响[J].南方医科大学学报,2013,(9):1285-1288,4.基金项目
国家自然科学基金 （）
