南方医科大学学报Issue(9)：1295-1298,4.DOI:10.3969/j.issn.1673-4254.2013.09.08
肝螺杆菌甲基基团趋化信号转导蛋白多克隆抗体的制备及鉴定
Preparation and identification of polyclonal antibody against methyl-accepting chemotaxis signal transduction protein of Helicobacter hepaticus
摘要
Abstract
Objective To prepare the polyclonal antibody against methyl-accepting chemotaxis signal transduction protein (MCP) of Helicobacter hepaticus (H.hepaticus). Methods The recombinant plasmid pET22b+/MCP was transformed into E.coli BL2l(DE3) to express the fusion protein His-rhMCP under the induction of IPTG. The fusion protein was purified and the antibody was obtained by immunizing rabbits. The titer of the polyclonal antibody was tested by indirect ELISA, and the specificity of the antibody was identified based on Western blotting using the prepared cell surface proteins (CSPs) of the bacteria. Results The fusion protein was successfully expressed, and the titer of the antibody reached 1∶32 000. Western blotting indicated that the antibody could specifically bind to CSPs and His-rhMCP. Conclusion The antibody with a high titer and specificity was prepared to facilitate further study of the pathogenicity and epidemiology of H.hepaticus in human.关键词
肝螺杆菌/甲基基团趋化信号转导蛋白/抗体制备Key words
Helicobacter hepaticus/signal transduction protein/antibody preparation引用本文复制引用
何胜平,陈雅华,赵瑛瑛,王继德,白杨..肝螺杆菌甲基基团趋化信号转导蛋白多克隆抗体的制备及鉴定[J].南方医科大学学报,2013,(9):1295-1298,4.基金项目
国家自然科学基金 （）
