南方医科大学学报Issue(9)：1304-1307,4.DOI:10.3969/j.issn.1673-4254.2013.09.10
3-溴丙酮酸诱导人乳腺癌SK-BR-3细胞凋亡及对细胞线粒体膜电位的影响
Effect of 3-bromopyruvate on mitochondrial membrane potential and apoptosis of human breast carcinoma SK-BR-3 cells
摘要
Abstract
Objective To study the effect of glycolysis inhibitor 3-bromopyruvate (3-BrPA) in inducing apoptosis of human breast carcinoma cells SK-BR-3 and the possible mechanism. Methods MTT assay was used to detect the growth inhibition induced by 3-BrPA in breast cancer cells SK-BR-3. The apoptotic cells were detected by flow cytometry with propidium iodide (PI). ATP levels in the cells were detected by ATP assay kit, and DHE fluorescent probe technique was used to determine superoxide anion levels; the mitochondrial membrane potential was assessed using JC-1 staining assay. Results MTT assay showed that the proliferation of SK-BR-3 cells was inhibited by 3-BrPA in a time- and concentration-dependent manner. Exposure to 80, 160, and 320μmol·L-1 3-BrPA for 24 h resulted in cell apoptosis rates of 6.7%, 22.3%, and 79.6%, respectively, and the intracellular ATP levels of SK-BR-3 cells treated with 80, 160, 320μmol·L-1 3-BrPA for 5 h were 87.7%, 60.6%, and 23.7%of the control levels. 3-BrPA at 160μmol·L-1 increased reactive oxygen levels and lowered mitochondrial membrane potential of SK-BR-3 cells. Conclusion 3-BrPA can inhibit cell proliferation, reduce the mitochondrial membrane potential and induce apoptosis in SK-BR-3 cells, the mechanism of which may involve a reduced ATP level by inhibiting glycolysis and increasing the reactive oxygen level in the cells.关键词
乳腺癌细胞/3-溴丙酮酸/糖酵解/ATP/活性氧Key words
breast cancer cells/3-bromopyruvate/glycolysis/ATP/reactive oxygen引用本文复制引用
张媛媛,刘哲,张倩雯,晁振华,张配,夏飞,蒋琛琛,刘浩,蒋志文..3-溴丙酮酸诱导人乳腺癌SK-BR-3细胞凋亡及对细胞线粒体膜电位的影响[J].南方医科大学学报,2013,(9):1304-1307,4.基金项目
国家自然科学基金（81000992，81072207）；安徽省自然科学基金（090413135）；安徽省高校省级自然科学研究重点项目（KJ2012A202） Supported by National Nature Science Foundation of China (81000992,81072207) （81000992,81072207）
