南方医科大学学报Issue(9)：1362-1366,5.DOI:10.3969/j.issn.1673-4254.2013.09.23
提高大鼠原位肝移植成功率的技术细节及围手术期处理
Surgical techniques and perioperative management for improving the success rate of orthotopic liver transplantation in rats
摘要
Abstract
Objective To investigate the surgical techniques and appropriate perioperative management for ensuring successful orthotopic liver transplantation (ROLT) in rats. Methods Based on the double-cuff technique of Kamada, we modified the surgical techniques of separation, perfusion and cold preservation of the donor liver, shearing and anastomosis of the suprahepatic vena cava with optimized postoperative infusion protocols and animal care. Results Two hundred and senventy rats underwent ROLT and a learning curve of the success rate was built to reflect the impovment of techniques. The learning curve showed steep improvemts over the exploration stage, breakthrough stage and maturation stage, and the success rates incresed sharply over time (0%, 71.1%, and 94.5%, respectively) until finnally reaching over 90%. The shearing and anastomosis of the suprahepatic vena cava remained the most critical and difficult techniques in ROLT modeling. Conclusion Proficient microsurgical techniques and meticulous nursing can reduce postoperative complications, enhance operational success rate and extend the survival time after ROLT.关键词
原位肝移植/大鼠/动物模型/无肝期Key words
Orthotopic liver transplantation/animal models/anhepatic phase引用本文复制引用
钟克波,杨定华,李湘竑,林梁,何华,梁建坤,刘潇,梁博,李嘉璐..提高大鼠原位肝移植成功率的技术细节及围手术期处理[J].南方医科大学学报,2013,(9):1362-1366,5.基金项目
广东省自然科学基金（10151051501000120）；广东省高等学校人才引进项目 （）
