福建畜牧兽医Issue(5)：1-5,5.
人血清白蛋白基因的克隆及其真核表达载体构建
Gene cloning of human serum albumin and the construction of eukaryotic expression vector
摘要
Abstract
About 1.8 kb sequence of the fragment of human serum albumin containing were successfully amplified by RT-PCR.The purpose of the initia gene were identified with the help of electrophoresis. The DNA fragment was cloned into vector pGEM-T and transformed into E.coli DH5a host bacteria. Xba Iendonuclease digestion and sequencingfrom selected positive cloned bacteria. The fragment was sequenced,and it was compared with serum albumin cDNA gene of humanin GenBank.The results indicated the homology were 97%.Using 5’-flanking of ovalbumin in quail for the control sequence to activate the expression of HAS.With restrictive interior contact enzyme kpnI with HindⅢ cuts material particle pOV and pHSA,then connects reorganization material particle for pOV-pHSA with the T4 ligase. The fusion vector was sequenced by enzyme digestion.关键词
人血清白蛋白/鹌鹑/卵管特异性表达载体Key words
human serum albumin/quail/oviduct-specific expressional vector引用本文复制引用
洪志勇,李玉芳,张兴峰,林秀娇,李鸿翔,庄益芬,张文昌..人血清白蛋白基因的克隆及其真核表达载体构建[J].福建畜牧兽医,2013,(5):1-5,5.基金项目
福建省自然科学基金项目（2007J0042）资助。 （）
