临床与病理杂志2015，Vol.35Issue(2)：243-247,5.DOI:10.3978/j.issn.2095-6959.2015.02.018
丹参联合黄芪对糖尿病肾病大鼠肾脏线粒体呼吸及能量代谢的影响
Protect effect of Salvia miltiorrhiza and APS on renal mitochondrial energy metabolism in rats with diabetic nephropathy
摘要
Abstract
Objective:To investigate the effects of Salvia miltiorrhiza and astragals polysaccharide (APS) on the mitochondrial respiration and energy metabolism in diabetic nephropathy rat.Methods: SD rats were randomly divided into DN group, Salvia miltiorrhiza group, APS group, union group and Normal group. Each group has 8 rats. DN group, Salvia miltiorrhiza group, APS group and union group were induced by STZ. Salvia miltiorrhiza group were given Salvia miltiorrhiza per day, APS group were given APS per day, union group were given Salvia miltiorrhiza + APS per day while the DN group and Normal group were given Normal Saline respectively for 6 weeks. The level of serum glucose, BUN, Cr and 24-hour urine albumin and urine volume were examined. HPLC was used to evaluate the content of ATP, ADP and AMP. Mitochondria were isolated with different centrifugations, the Clark electrodes were used to test the mitochondrial T3, T4 and RCR.Results:Compared with DN group, the serum glucose, BUN, Cr , 24-hour urine albumin were lower in Salvia miltiorrhiza group, APS group and union group. The 24-hour urine volume, the content of ATP and ADP of kidney, the mitochondrial T3 and RCR were higher in Salvia miltiorrhiza group, APS group and union group than those in DN group with statisticaly difference. There is no statistically difference between union group and Salvia miltiorrhiza (or APS group) group.Conclusion: Salvia miltiorrhiza and APS may provide protection by improving the mitochondrial respiration and energy metabolism in diabetic nephropathy. Salvia miltiorrhiza + APS treatment has no advantage than single medicine.关键词
丹参/黄芪/糖尿病肾病/线粒体/能量代谢Key words
Salvia miltiorrhiza/astragals polysaccharide/diabetic nephropathy/mitochondria/energy metabolism引用本文复制引用
王晓华,何杏容,华红艳,王志锋..丹参联合黄芪对糖尿病肾病大鼠肾脏线粒体呼吸及能量代谢的影响[J].临床与病理杂志,2015,35(2):243-247,5.基金项目
广州市番禺区科技计划项目(2010-Z-94-1).This work was supported by the Panyu Technique Foundation (2010-Z-94-1), P. R. China. （2010-Z-94-1）
