广西医学2015，Vol.37Issue(3)：292-295,4.DOI:10.11675/j.issn.0253-4304.2015.03.02
二甲双胍对2型糖尿病伴非酒精性脂肪肝大鼠的治疗作用研究
Effect of metformin on non-alcoholic fatty liver disease in typeⅡdiabetic rats
摘要
Abstract
Objective To observe the effects of metfomin on liver function and hepatic pathological morphology in rats with typeⅡdiabetes mellitus( T2DM) and non-alcoholic fatty liver disease( NAFLD) ,and to explore its possible mechanism.Methods Thirty-six Wistar rats were randomly divided into normal group(n=12) and model group(n=24).The rat model of T2DM and NAFLD was established by the intraperitoneal injection with a small dose of streptozotocin( STZ) and the fat-rich food feeding.The rats in model group were randomly divided into single model group(n=14) and metformin group(n=10).The levels of serum alanine aminotransferase (ALT),aspartate aminotransferase(AST),triglyceride(TG),cholesterol(CHO),low density lipoprotein cholesterol(LDL-C) of rats were compared among all groups,as well as the levels of TG,CHO,superoxide dismutase(SOD),catalase(CAT),nitric oxide(NO) and nitric oxide synthase ( NOS) in liver tissues.Results Compared with normal group,the levels of serum ALT,AST,TG,CHO and LDL-C increased significantly in the model group(P<0.05),as well as the levels of TG,CHO,NO and NOS in liver tissues(P<0.05),and the levels of SOD and CAT in liver tissues decreased(P<0.05).The metformin group showed significantly lower levels of serum ALT,AST,TG,CHO and LDL-C compared with single model group(P<0.05),as well as the levels of TG,CHO,NO and NOS in liver tissues(P<0.05),and the levels of SOD and CAT in liver tissues increased in metformin group(P<0.05).Conclusion The fatty liver of T2DM rat with NAFLD can be significantly improved by metformin treatment.The mechanism may be related with the improvement of oxidative stress in liver tissues by metformin.关键词
2型糖尿病/非酒精性脂肪肝/二甲双胍/氧化应激Key words
Type 2 diabetes mellitus/Non-alcoholic fatty liver disease/Metformin/Oxidative stress分类
医药卫生引用本文复制引用
YAN Mei-hua,李忻,顾立刚,赵婷婷,张韫,杨鑫,张浩军,严美花,董晞,赵海玲..二甲双胍对2型糖尿病伴非酒精性脂肪肝大鼠的治疗作用研究[J].广西医学,2015,37(3):292-295,4.基金项目
国家自然科学基金(81173422) （81173422）
国家国际科技合作专项(2011DFA31860) （2011DFA31860）
