黑龙江农业科学Issue(9)：17-21,27,6.
紫薇SSR-PCR反应体系优化和引物筛选
Optimization of SSR-PCR Reaction System and Primers Screening of Lagerstroemia indica L .
摘要
Abstract
In order to further research the molecular markers of L agerstroemia indica L .,taking DNA of L a-gerstroemia indica L .as template ,several important parameters influencing SSR-PCR amplification were stud-ied with an orthogonal design by four factors and three levels ,as to establish the optimum SSR-PCR reaction system ,and the primers were screened from 24 pairs of SSR primers .The results showed that the optimal SSR-PCR reaction system was established as a total volume of 10 μL including Taq DNA polymerase 0 .2 U ,Mg2+2 .5 mmol·L-1 ,dNTPs 0 .15 mmol·L-1 ,0 .25 mmol·L-1 of each primer and template DNA 60 ng ,deionized water to 10 μL .23 pairs of available primers were screened from 24 pairs of EST-SSR primers ,there were 16 pairs of polymorphism primers amplified out 68 clear bands ,39 bands with differences .关键词
紫薇/SSR-PCR/体系优化/引物筛选Key words
L agerstroemia indica L ./SSR-PCR/system optimization/primers screening分类
农业科技引用本文复制引用
王鹏,张振宇,马玲玲,马育珠,杨如同,李亚..紫薇SSR-PCR反应体系优化和引物筛选[J].黑龙江农业科学,2013,(9):17-21,27,6.基金项目
江苏省农业自主创新资金资助项目［CX （11）1039］；江苏省科技公共服务平台资助项目（BM2012058）；江苏省农业三新工程资助项目［SXGC（2012）412］；南京市现代农业生产技术资助项目（201201021）；江苏省自然科学基金资助项目 （）
