黑龙江农业科学Issue(9)：45-48,4.
氯嘧磺隆降解菌F31发酵条件及降解能力的研究
Study on Fermentation Conditions and Degradation Ability of Degrading Fungi F31 of Chlorimuron-ethyl
摘要
Abstract
In order to promote and accelerate industrialization of degrading fungi ,solve the residual phytotoxicity of chlorimuron-ethyl ,with the single factor screening and orthogonal test ,fermentation of different carbon sources ,nitrogen sources and inorganic chemicals were systematically studied with index of degrading fungi growth(OD600 ) .The results showed that in the single factor test ,carbon source glucose ,nitrogen source pep-tone and (NH4 )2SO4 ,inorganic salts KH2 PO4 ,NaCl ,MgSO4 and FeSO4 were primarily screened out ,which were benefit for the growth of the degrading fungi .Based on the single factor test ,optimal carbon sources ,ni-trogen sources and inorganic chemicals were chosen to be component factors ,and each of them was operated with orthogonal optimization test by three levels ,thereby achieving optimum culture medium formula which was 1% glucose ,2% peptone ,1% (NH4 )2 SO4 ,0 .07% KH2 PO4 ,0 .2% NaCl ,0 .07% MgSO4 and 0 .000 5%FeSO4 .The best medium shake flask tests showed that F31 could grow well in the new medium and the degra-dation ability was strong ,chlorimuron-ethyl with HPLC wasn’t detected after 36 h .关键词
氯嘧磺隆/降解真菌F31/摇瓶培养/降解能力Key words
chlorimuron-ethyl/degrading fungi F31/shake flask/degradation ability分类
农业科技引用本文复制引用
任洪雷,陶波,李春霞..氯嘧磺隆降解菌F31发酵条件及降解能力的研究[J].黑龙江农业科学,2013,(9):45-48,4.基金项目
哈尔滨市科技攻关资助项目 （）
