河南农业科学Issue(10)：25-30,6.
KLU基因转化亚麻荠的初步研究
Preliminary Study on Transforming KLU into Camelina sativ a (L .) Crantz
摘要
Abstract
For further improving the oil content of an alternative oil crop species Camelina sativ a (L .) Crantz ,we cloned the Arabidopsis ovule organ-specific promoter (pINO) and the Arabi-dopsis cytochrome P450 KLUH gene (KLU) .Next ,the plant expression vector pCAMBIA2301 was digested and fused KLU with pINO to construct the plant express vector pCAMBIA 2301-pI-NO-KLU ,which was introduced into Camelinasativa (L .) Crantz with Agrobacterium-mediated floral dip transformation .The results showed that through using the Agrobacterium pellet to transform Camelina sativa (L .) Crantz ,which was resuspended by the same volume of infiltra-tion medium (1/2MS ,5% sucrose ,200 μL/L Silwet L-77) when Agrobacterium culture was cul tured to OD600 =0 .8 ,positive rate of screening transgenic seeds by Kan (50 mg/L) was 15% .Fur-ther PCR analysis confirmed that KLU gene had been incorporated into the genome of parts of re-sistant plant ,and the transformation efficiency was 1 .8% .关键词
亚麻荠/KLU/根癌农杆菌/滴花法Key words
Camelina sativ a (L .) Crantz/KLU/Agrobacterium time faciens/floral dip分类
农业科技引用本文复制引用
高立虎,蔡永智,王爱英,沈海涛,祝建波..KLU基因转化亚麻荠的初步研究[J].河南农业科学,2013,(10):25-30,6.基金项目
国家转基因专项（2011ZX005004）；兵团博士基金项目 （）
