海洋科学Issue(8)：1-5,5.
紫扇贝、海湾扇贝及其正反杂交子代群体遗传结构的AFLP分析
AFLP analysis of genetic structure of Argopecten purpuratus, Argopecten irradians and their positive and negative hybrid offspring
摘要
Abstract
The AFLP molecular marker technique was used to analyze the genetic structure of Argopecten purpu-ratus (PP), Argopecten. irradians (II) and their hybrid offspring (A. purpuratus♀×A. irradians♂, PI;A. irradians♀×A. purpuratus♂, IP) in order to reveal the genetic differences and relationships between the parents and their offspring. Five AFLP primers were used to amplify the genome DNA, and 433 loci were yielded, the polymorphic loci of which accoutered for 88.45%. The majority of the amplified loci (97.23%) of the hybrid offspring were in-herited from both the parents, indicating that the hybrids were real heterogenous. Loci which not follow the Mende-lian Law were found in the analysis that some of the loci of the A. purpuratus and A. irradians were lost and 12 new loci were amplified in the hybrid offspring. It was revealed from the analysis of the percentage of polymorphic loci, Nei’s gene diversity, Shannon’s diversity index, genetic distance and AMOVA that the genetic differentiation be-tween the positisve and negative hybrid offspring was smaller than that between the offspring with their parents.关键词
紫扇贝(Argopecten purpuratu)/海湾扇贝(Argopecten irradians)/杂交/AFLPKey words
Argopecten purpuratus/Argopecten. irradians/hybrid offspring/AFLP分类
农业科技引用本文复制引用
孙妍,黄晓婷,胡丽萍,王师,王春德,包振民..紫扇贝、海湾扇贝及其正反杂交子代群体遗传结构的AFLP分析[J].海洋科学,2013,(8):1-5,5.基金项目
国家高技术研究发展计划(2012AA10A410) （2012AA10A410）
国家自然科学基金项目(31172404) （31172404）
国家科技支撑计划项目(2011BAD45B01) （2011BAD45B01）
现代农业产业技术体系建设专项资金资助项目(CARS-48) （CARS-48）
