海洋科学Issue(8)：67-71,5.
栉江珧精子超微结构的电镜观察
Observation on ultrastructure of spermatozoon in Atrina pectinata with electron microscope
摘要
Abstract
To provide the cytology basis for the mechanism of Atrina pectinata fertilization, the ultrastructure of spermatozoon in A. pectinata was observed under transmission electron microscopy (TEM). The results showed that the mature spermatozoon of A. pectinata was typical primitive type, composed of 3 parts including head, middle piece and tail. The head is egg-shaped, and it is composed of acrosome and nucleus. The acrosome is upside-down V-shaped. The distribution of acrosomal materials is uneven. There is an arc near the outer acrosomal membrane with much higher electron density than that of other areas. The nucleus is mainly made up of highly enriched chro-matin, but lower electron density irregular structure is also found. Implantation fossa and anterior nuclear fossa can be observed. However, posterior nuclear fossa is absent. The centriolar complex which is comprised of 2 orthogonal centrioles and 5 surrounding oval mitochondria constitute the middle piece of spermatozoon. Mitochondrion, formed by 2 membranes, is almost spherical in shape. Flagellum in tail is constituted by wavy plasma membrane and axoneme. These results showed that the ultrastructure of spermatozoon in A. pectinata is structurally similar to the typical bivalve spermtozoon, and illustrate the fertilization mechanism of A. pectinata to some extent.关键词
栉江珧(Atrina pectinata)/精子/超微结构/透射电镜Key words
Atrina pectinata/spermatozoon/ultrastructure/transmission electron microscopy分类
生物科学引用本文复制引用
顾向飞,边平江,周小龙,林志华,董迎辉..栉江珧精子超微结构的电镜观察[J].海洋科学,2013,(8):67-71,5.基金项目
国家现代贝类产业技术体系项目(CARS-48) （CARS-48）
宁波市科技创新团队项目(2011B82017) （2011B82017）
宁波市科技局农业择优委托项目(2010C10011) （2010C10011）
