井冈山大学学报（自然科学版）Issue(4)：37-40,4.DOI:10.3969/j.issn.1674-8085.2013.04.008
匍匐茎草本蛇莓RAPD反应体系优化
OPTIMIZATION OF RAPD REACTION SYSTEM IN THE STOLONIFEROUS HERB, DUCHESNEA INDICA
摘要
Abstract
In order to establish the optimal RAPD reaction system in Duchesnea indica, every factor in the system was optimized. Based on the RAPD reaction system commonly used in our lab, the optimal RAPD reaction system of genome DNA of D. indica was determined according to the RAPD amplification effects. The optimal PCR system was as follows:2.5μL 10×buffer, 2.0 mmol/L Mg2+, 1.5 U Taq polymerase, 50 ng DNA template, 0.2 mmol/L dNTPs, 0.35μmol/L random primer S30 in 25μl reaction system. The results of this paper will lay the basis for assessing the genetic diversity of the wild D. indica.关键词
蛇莓/RAPD/扩增程序Key words
Duchesnea indica/RAPD/amplification procedure分类
生物科学引用本文复制引用
廖信军,李雅宜,胡雪华..匍匐茎草本蛇莓RAPD反应体系优化[J].井冈山大学学报（自然科学版）,2013,(4):37-40,4.基金项目
井冈山大学科研项目(JZ0821) （JZ0821）
