检验医学与临床Issue(14)：1808-1809,1811,3.DOI:10.3969/j.issn.1672-9455.2013.14.015
高效液相色谱分析在HbE筛查中的应用价值
Application value of high performance liquid chromatography in the screening for HbE disease
李东明 1玉晋武 1韦媛 1王立芳 1张强 1阙婷 1唐燕青 1何升1
作者信息
- 1. 广西壮族自治区妇幼保健院遗传代谢中心实验室,南宁,530003
- 折叠
摘要
Abstract
Objective To explore the value of high performance liquid chromatography in the screening for HbE disease .Methods 24 779 samples were detected for hemoglobin by high performance liquid chromatography , and HbA2≥ 11% samples for genetic testing of HbE .Results Among 24 779 samples identifying 129 samples with HbA2≥11% ,the content was 14 .0% -75 .2% (27 .2% ± 9 .4% ) .Genetic testing were diagnosed as HbE disease ,in-cluding HbE heterozygous ,HbE/α-thalassemia ,HbE/β-thalassemia homozygotes ,HbE composited alpha and beta thalassemia and HbE homozygotes ,the HbA2 of which were (26 .1 ± 1 .3)% ,(18 .2 ± 1 .8)% ,(45 .9 ± 8 .0) ,38 .6%and (73 .9 ± 1 .8)% .The cutoff value of HbA2 in the screening for HbE disease was 11 .7% by ROC curve analysis . Conclusion High performance liquid chromatography is an effective screening method for HbE disease ,which can distinguish between HbE disease mutation type and composite thalassemia by HbA 2 content ,is an effective method for screening HbE disease .关键词
高效液相色谱/HbE/筛查/珠蛋白生成障碍性贫血Key words
high performance liquid chromatography/HbE/screening/thalassemia引用本文复制引用
李东明,玉晋武,韦媛,王立芳,张强,阙婷,唐燕青,何升..高效液相色谱分析在HbE筛查中的应用价值[J].检验医学与临床,2013,(14):1808-1809,1811,3.
