昆明医科大学学报Issue(8)：31-35,5.
重组表达hTFF2促进结肠癌细胞迁移和失巢增殖作用
Recombinant Expression of hTFF2 and Its Roles in Migration and Anchorage-independent Growth of Colonic Cancer Cells
摘要
Abstract
Objective To over-express human trefoil factor 2 (hTFF2) by Escherichia coli system and an-alyze its activities in promoting migration and anchorage-independent growth in SW480 colonic cancer cells. Meth-ods hTFF2 gene encoding mature peptide was obtained by RT-PCR, and the recombinant expression vector pET32a-hTFF2 was constructed. Then pET32a-hTFF2 was transformed into E. coli BL21-32a and TrxA-hTFF2 fu-sion protein was induced to over-express. The expressed product was isolated by Ni-NTA affinity chromatography, purified by dialysis and identified by Western blotting. The activities of the recombinant hTFF2 in promoting SW480 cells migration and anchorage-independent growth were analyzed by MicroChemotaxis Chamber migration assay and Soft-agar assay,respectively. Results The TrxA-hTFF2 fusion protein was expressed to 220 mg/L at high purity. In vitro model demonstrated that recombinant hTFF2 obviously enhanced SW480 cell migration activity and anchor-age-independent growth. Conclusion The recombinant hTFF2 can be expressed in E. coli with high production, purity and biological activities. And its roles in cell migration and anchorage-independent growth suggest that up-regulation of TFF2 in colonic cancer might be involved in cancer invasion and metastases.关键词
三叶因子2/结肠癌/细胞迁移/失巢增殖Key words
Trefoil factor 2/Colonic cancer/Cell migration/Anchorage-independent growth分类
医药卫生引用本文复制引用
蒋伟国,罗方梅,王皓颖,谢薇,秦钰,黄尤光..重组表达hTFF2促进结肠癌细胞迁移和失巢增殖作用[J].昆明医科大学学报,2013,(8):31-35,5.基金项目
云南省科技计划项目 （）
