泸州医学院学报Issue(4)：325-327,3.DOI:10.3969/j.issn.1000-2669.2013.04.003
卵母细胞双电极电压钳技术的建立及其记录到的内源性外向电流*
Establishment of two-microelectrode voltage-clamp technique for oocytes and the recorded endogenous outward currents
摘要
Abstract
Objective: To establish two-microelectrode voltage-clamp technique for oocytes. Methods: Two-microelectrode voltage-clamp technique platform was established and the endogenous outward ionic currents were recorded on bullfrog oocytes after the removal of follicular theca. Results: As the oocytes were incubated in normal ND96 solution, the membrane potential was maintained at -80 mV, then depolarized to +50 mV in 10 mV increments, the outward cuttents were recorded. After applied 10 mmol/L TEA in the extracellular fluid, more than 80% currents were blocked, then recovery to about 90% after washout. The currents did not obviously change in ND96 solution without calcium. Conclusion: Two -microelectrode voltage -clamp technique is successfully established in oocytes. The outward currents induced by depolarizing stimulation on bullfrog oocytes were potassium currents.关键词
牛蛙卵母细胞/内源性外向电流/双电极电压钳/TEAKey words
Bullfrog oocytes/Endogenous outward currents/Two-microelectrode voltage clamp/TEA分类
医药卫生引用本文复制引用
王娜,杨艳,兰欢,毛亮,陈唐葶,曾晓荣..卵母细胞双电极电压钳技术的建立及其记录到的内源性外向电流*[J].泸州医学院学报,2013,(4):325-327,3.基金项目
国家自然科学基金资助项目 （）
