热带亚热带植物学报Issue(5)：406-413,8.DOI:10.3969/j.issn.1005-3395.2013.05.003
福建省野牡丹属种质资源的ISSR分析
ISSR Analysis of Melastoma Germplasm in Fujian Province
摘要
Abstract
In order to understand the relationship of Melastoma, the DNA ifngerprints of 34 Melastoma germplasm collected from different regions of Fujian Province were studied by using ISSR markers. Eleven primers selected from 116 ISSR primers could obtain high polymorphism and reproducibility bands. The results showed that total of 112 DNA bands were ampliifed, and 104 polymorphic bands, counting for 92.9%. Genetic similarity analysis showed that for 34 Melastoma germplasm the Nei’s coefifcient of 34 Melastoma germplasm ranged from 0.55 to 0.93, with the average of 0.71. It was suggested that there were close genetic relationships and high genetic diversity among Melastoma germplasm resources. These 34 germplasm resources could divided into 3 groups and 5 subgroups by UPGMA analysis, and 4 groups by principal coordinate scatter analysis. These results had high consistency with that of genetic relationship analysis. Therefore, it was suggested that ISSR markers could be used to reveal genetic diversity of Melastoma at molecular level, and provided a theoretical basis for breeding of Melastoma in Fujian.关键词
野牡丹属/亲缘关系/分子标记/种质资源/福建省Key words
Melastoma/Genetic relationship/Molecular marker/Germplasm/Fujian Province引用本文复制引用
郑涛,陈振东,林秀香,郑少缘,何雪娇,苏金强..福建省野牡丹属种质资源的ISSR分析[J].热带亚热带植物学报,2013,(5):406-413,8.基金项目
福建省自然科学基金项目(2011J01122) （2011J01122）
福建省公益类科研专项(2011R030-1)资助 （2011R030-1）
