热带亚热带植物学报Issue(5):414-419,6.DOI:10.3969/j.issn.1005-3395.2013.05.004
扇脉杓兰和无距虾脊兰的核型分析
Karyotype Analysis of Cypripedium japonicum Thunb. and Calanthe tsoongiana T. Tang et F. T. Wang
摘要
Abstract
The aim was to understand the karyotype of Cypripedium japonicum Thunb. and Calanthe tsoongiana T. Tang et F. T. Wang. Their chromosome number and karyotype were studied by using ifngertip squashing method. The results showed that the chromosome number of Cypripedium japonium was 2n = 22, karyotype formula was 2n=2x=22=16m+2sm+2st+2t, constitution of relative length was 2n=22=2L+6M2+12M1+2S, asymmetry index was 60.01%, karyotype type was classiifed as 2B;while the chromosome number of Calanthe tsoongiana was 2n=40, karyotype formula was 2n=2x=40=28m+10sm+2st, constitution of relative length was 2n = 40 = 8L+ 10M2+ 16M1+ 6S, asymmetry index was 59.84%, karyotype type also belonged to 2B. Both of them have relatively symmetric karyotype. The karyotype of Calanthe tsoongiana was reported for the first time. These provided some cytological evidences for evolutionary status and germplasm conservation of Cypripedium japonicum and Calanthe tsoongiana.关键词
扇脉杓兰/无距虾脊兰/染色体/核型Key words
Cypripedium japonicum/Calanthe tsoongiana/Chromosome/Karyotype引用本文复制引用
钱鑫,连静静,李全健,刘芬,王彩霞,田敏..扇脉杓兰和无距虾脊兰的核型分析[J].热带亚热带植物学报,2013,(5):414-419,6.基金项目
浙江省省院合作林业项目(2012SY02-3)资助 (2012SY02-3)