水生生物学报2015，Vol.39Issue(3)：459-467,9.DOI:10.7541/2015.61
斑马鱼fem-1c cDNA克隆与表达分析
THE CLONING AND EXPRESSION ANALYSIS OF ZEBRAFISHFEM-1C, A MEMBER OFFEM-1 FAMILY
摘要
Abstract
Feminization-1 (fem-1) gene was first discovered in Caenorhabditis elegans, and itplays an important role in nematode sex determination. The mutation infem-1c could feminize the nematode sex differentiation. There are three conservative members offem-1 family in vertebrate, namely fem-1a,fem-1bandfem-1c. We cloned the entire cDNA sequence offem-1cfrom zebrafish ovary cDNA library using RACE-PCR. The full length of zebrafishfem-1c cDNA was 2701 bp, encoding a 618-amino acid protein. We used online bioinformatic software and predicted that the FEM-1C protein contained 9 ANK domains, 2 TPR domains and 2 low complexity regions. Zebrafish FEM-1C shared a highly conservative protein sequence with its homologues in other vertebrates. The sequence analysis also revealed that in vertebrates there was a conservative synteny betweenfem-1c and its neighborhood genes, especially the nearest 4—5 genes such as tmed7 andtrim36. We conducted the expression analysis in different tissues of adult zebrafish using RT-PCR and found thatfem-1c was exclusively expressed in the ovary. The expression analysis in the gonad of larvae at different development stages showed that the expression offem-1c began on the 17th day post-fertilization. Thein situ hybridization results showed thatfem-1c was expressed in cytoplasm at stage I andⅡ of oocyte. This specific expression pattern suggested thatfem-1c might play a role in zebrafish ovary development.关键词
fem-1c/卵巢分化/原位杂交/斑马鱼Key words
fem-1c/Ovary differentiation/In situ hybridization/Zebrafish分类
生物科学引用本文复制引用
侍建涛,李志,桂建芳,周莉..斑马鱼fem-1c cDNA克隆与表达分析[J].水生生物学报,2015,39(3):459-467,9.基金项目
国家973计划(2010CB126301) （2010CB126301）
现代农业产业技术体系建设专项资金(NYCYTX-49)资助 （NYCYTX-49）
