特产研究Issue(3)：61-64,4.
高效液相法测定万寿菊颗粒中叶黄素的含量
HPLC Determination of the Content of Lutein Marigold Particles
摘要
Abstract
Ultrasound ,reflux and soaking methods were used for separation of lutein marigold extract and then purified by liquid saponification its determination .The best method of separation and extraction was chosen.ODS C18 column using filler(4.6 × 250mm ,5μm) ,Column temper-ature at 30℃ ,flow at 1mL/min ,sample 10μL ,time:22min ,mobile phase A (acetonitrile∶methanol=9∶1 )∶B (ethyl acetate ) .Samples using a gradient elution ,0~5min when B Mobile phase at 10% ,5~15min when B Mobile phase was set at 15% ,15~20min when B Mobile phase was 0% ,the detection wavelength was at 450nm .Soaked by the lutein extraction yield can be seen as the extraction of lutein marigold particles effective experimental methods and soak 16h marigold lutein yield most .Lutein concentration and peak area ,respectively 0.1~0.6mg/mL( r=0.999 7 ) showed good linearity .Standards and samples by liquid chromatogram determined by comparing the sample obtained after saponifica-tion of lutein .This experiment proved the best lutein extraction saponification experiments .关键词
万寿菊/叶黄素/高效液相色谱Key words
Tagetes erecta L ./lutein/HPLC分类
医药卫生引用本文复制引用
王睿,孙德亚,王艳,刘昕..高效液相法测定万寿菊颗粒中叶黄素的含量[J].特产研究,2013,(3):61-64,4.基金项目
吉林省科技发展计划医药产业发展专项资金 （）
