中国组织工程研究Issue(36)：6423-6429,7.DOI:10.3969/j.issn.2095-4344.2013.36.007
去卵巢骨质疏松大鼠骨髓间充质干细胞的体外成骨诱导
In vitro osteogenic differentiation of bone marrow mesenchymal stem cells from ovariectomied osteoporotic rats
摘要
Abstract
BACKGROUND:Cytological studies show that bone marrow mesenchymal stem cel s play an important role in postmenopausal osteoporosis mechanism. <br> OBJECTIVE:To study the osteogenic differentiation in vitro of bone marrow mesenchymal stem cel s from ovariectomied osteoporotic rats. <br> METHODS:The osteoporotic animal model was established by performing ovariectomy in the 6-month-old female Sprague-Dawley rats. There were four groups:bone marrow mesenchymal stem cel s control group, bone marrow mesenchymal stem cel s osteoporosis group, bone marrow mesenchymal stem cel s osteogenic induction group and oseogenesis induction group. Bone marrow mesenchymal stem cel s were isolated from the rats of control group and oseogenesis induction group by means of the whole bone marrow adherence method and cultured to the 3rd generation. Then the bone marrow mesenchymal stem cel s were used in al the experiments. Cel morphology was observed under the inverted phase contrast microscope, cel cycle and proliferation index of bone marrow mesenchymal stem cel s were detected by flow cytometry. After osteogenic induction, the expression level of alkaline phosphatase was detected, and the fornation of calcium nodes of bone marrow mesenchymal stem cel s were marked by alizarin red staining. <br> RESULTS AND CONCLUSION:The cel s in the osteogenic induction group and oseogenesis induction group had the morphology of osteobalsts, and the change of morphology of the cel s in the oseogenesis induction group was relatively tardiness. The proliferation index in the control group was higher than that in the osteoporosis group (P<0.05);expression level of alkaline phosphatase in the osteogenic induction group was significantly higher than that in the oseogenesis induction group (P<0. 05), and the control group was significantly higher than the oseogenesis group (P<0.05). The alizarin red staining of the cel s in the osteogenic induction group was positive, while negative in the control group and the oseogenesis group;the staining in the osteogenic induction group was stronger than that in the oseogenesis induction group. These findings indicate that both the proliferative potential and the osteogenic potential of bone marrow mesenchymal stem cel s from the ovariectomized osteoporotic rats are decreased, which may be related with the ostoeporosis pathogensis of ovariectomied rats.关键词
干细胞/骨髓干细胞/骨髓间充质干细胞/骨质疏松/绝经/去卵巢/成骨诱导/碱性磷酸酶/细胞周期/增殖指数/省级基金/干细胞图片文章分类
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王云,鲍小明,侯永新,李军,张民..去卵巢骨质疏松大鼠骨髓间充质干细胞的体外成骨诱导[J].中国组织工程研究,2013,(36):6423-6429,7.基金项目
山西省科技攻关项目(20100311098-2)@@@@Key Science and Technology Project of Shanxi Province, No.20100311098-2 （20100311098-2）
