中国组织工程研究Issue(36)：6430-6435,6.DOI:10.3969/j.issn.2095-4344.2013.36.008
储运条件对脐带间充质干细胞存活率的影响
Effects of transportation conditions on survival rate of umbilical cord mesenchymal stem cells
摘要
Abstract
BACKGROUND:General assessment of viability of umbilical cord mesenchymal stem cel s during transport is not considered at home and abroad. <br> OBJECTIVE:To explore the effects of different transportation conditions such as albumin and transport time on survival rate of umbilical cord mesenchymal stem cel s. <br> METHODS:Umbilical cord mesenchymal stem cel s cultured in vitro were divided into experimental and control groups. Each of group contained 3.15×109/L cel s in 3 mL normal saline. The experimental group contained 1%albumin in dark environment. The control group included two subgroups:dark preservation group with the absence of albumin and light preservation group with the presence of albumin. Cel counting, trypan blue staining and cel survival rate were compared at 0, 2, 4, 8, and 24 hours. <br> RESULTS AND CONCLUSION:Compared with the control group, no cel loss was observed in experimental group (with the presence of albumin in the dark) and a 90%viability ratio was achieved at 8 hours. In the control group without albumin, loss rate reached 38.5%and survival rate reached 86%at 8 hours. Results revealed that 1%albumin predominantly improved cel survival rate in long-distance transport of umbilical cord mesenchymal stem cel s. When transport time was more than 8 hours, cel loss rate increased and cel survival rate downregulated. Our experimental data demonstrated that umbilical cord mesenchymal stem cel s preserved in normal saline consisting of 1%albumin placing in dark environment at 16 ℃ apply to clinical application criterion in 8 hours.关键词
干细胞/脐带脐血干细胞/脐带/间充质干细胞/存活率/白蛋白/光/细胞培养/省级基金/干细胞图片文章分类
医药卫生引用本文复制引用
汪大琨,王芝辉,郭红,张乐玲..储运条件对脐带间充质干细胞存活率的影响[J].中国组织工程研究,2013,(36):6430-6435,6.基金项目
山东大学自主创新基金(2012ZD023)；山东省科技发展计划项目(2010G0020213)@@@@the Autonomous Innovation Project of Shandong University, No.2012ZD023 （2012ZD023）
the Shandong Provincial Technology Development Project, No.2010G0020213 （）
