中国组织工程研究Issue(36)：6515-6520,6.DOI:10.3969/j.issn.2095-4344.2013.36.021
黄芪甲苷联合丹参酮Ⅱa诱导骨髓间充质干细胞向心肌样细胞分化
Combination of tanshinone IIa and astragaloside induces bone marrow mesenchymal stem cells differentiating into myocardium-like cells
摘要
Abstract
BACKGROUND:Astragaloside and tanshinone IIa are the effective components of traditional Chinese medicine treatment of myocardial ischemia, and its role in bone marrow mesenchymal stem cel s differentiation into myocardium-like cel s remains unclear. <br> OBJECTIVE:To investigate the effect of tanshinone IIa and astragaloside on the differentiation of bone marrow mesenchymal stem cel s into myocardium-like cel s. <br> METHODS:The maximal non-toxic concentrations of tanshinone IIa and astragaloside were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, to define the dose of the two in the induced differentiation of bone marrow mesenchymal stem cel s into myocardium-like cel s. The isolated and purified bone marrow mesenchymal stem cel s were divided into five groups:astragaloside group, tanshinone IIa group, astragaloside+tanshinone IIa group, 5-azacitidine group, and blank control group. The expression of gap junction connexin 43 and troponin was determined with enzyme-linked immunosorbent assay. <br> RESULTS AND CONCLUSION:The expression of gap junction connexin 43 and troponin in astragaloside group, tanshinone IIa group, astragaloside+tanshinone IIa group, 5-azacitidine group was higher than that in blank control group (P<0.01). The astragaloside+tanshinone IIa group showed a higher expression of gap junction connexin 43 and troponin than astragaloside group and tanshinone IIa group (P<0.01). There was no significant difference in the expression of gap junction connexin 43 and troponin between astragaloside+tanshinone IIa group and 5-azacitidine group (P>0.05). A combined use of astragaloside and tanshinone IIa can induce bone marrow mesenchymal stem cel s to differentiate into myocardium-like cel s, and their joint role is better than the role of a single ingredient.关键词
干细胞/干细胞与中医药/丹参酮Ⅱa/黄芪甲苷/骨髓间充质干细胞/心肌样细胞/诱导分化/有效部位/心肌缺血/国家自然科学基金分类
医药卫生引用本文复制引用
宋雪云,龙云,谭超,郑景辉..黄芪甲苷联合丹参酮Ⅱa诱导骨髓间充质干细胞向心肌样细胞分化[J].中国组织工程研究,2013,(36):6515-6520,6.基金项目
国家自然科学基金项目(81102595)《养心通脉有效部位方对BMSCs作用靶点及信号转导通路的研究》；广西自然科学基金项目(2012GXNSFAA053113)《心血瘀阻证心肌微环境变化及对骨髓间充质干细胞移植的影响》@@@@the National Natural Science Foundation of China, No.81102595 （81102595）
the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No.2012GXNSFAA-053113 （）
