中国组织工程研究Issue(37)：6587-6593,7.DOI:10.3969/j.issn.2095-4344.2013.37.008
长期置入骶神经根刺激电极的兔组织学变化及其安全性
Histological changes and safety of long-term acral nerve root stimulation electrode placement in rabbits
摘要
Abstract
BACKGROUND:Studies have shown that sacral nerve-root stimulation based on anodes block technique can effectively reconstruct the bladder voiding function of the rabbits with spinal cord injury. But the corresponding technology of stimulating electrode has not been reported so far. <br> OBJECTIVE:To design and develop the stimulating electrodes matching with both rabbit sacral nerve roots and anodal blocking technique, to observe the ultrastructure and morphological change of rabbit sacral nerve roots which implanted in electrode stimulation for a long-term and to assess the safety of stimulating electrodes. <br> METHODS:Thirty New Zealand rabbits were included, 10 rabbits were randomly selected from them and sacrificed after anesthesia, and then cut the anterior roots of bilateral S 2 and S 3 immediately;after measuring the diameter under the light microscope, the sleeve type stimulation electrode matched with the diameter was made. The remaining 20 rabbits were randomly divided into control group and implantation group, with 10 rabbits in each group. In the implantation group, the stimulating electrodes were implanted into the forepart of S 2 and S 3 nerve roots after anesthesia, and then sacrificed after fed for half a year for col ecting the samples. Then ultrastructure change of sacral nerve roots with the implantation was observed. <br> RESULTS AND CONCLUSION:Structure of nerve cel s of sacral nerve roots remained in good condition under a light microscope after long-term implantation of the stimulating electrodes. No obvious degeneration of axons, no inflammatory infiltration and glial scar formation were observed. In the implantation group, myelins arranged closely without demyelination phenomenon, and there was no atrophy of neuronal nuclear, no nuclear sag, no increased nuclear decompression and heterochromatin in neurons under the light microscope. Immunohistochemical analysis showed, compared with the control group, there were no significant differences in the expressions of glial fibril ary acidic protein, Bax, Bcl-2 and Caspase-3 proteins of nerve roots in the implantation group. The stimulation electrode of rabbit sacral nerve root is developed successful y, that is, the implantation is simple and safe as it can be used for long-term implantation without histopathological changes and apoptosis.关键词
组织构建/神经组织构建/骶神经根/刺激电极/骶神经/髓鞘/应用/细胞凋亡/省级基金分类
医药卫生引用本文复制引用
闫鹏,郑伟东,张季凯,谭云冰,李高峰,李光淳,宋成,杨小玉..长期置入骶神经根刺激电极的兔组织学变化及其安全性[J].中国组织工程研究,2013,(37):6587-6593,7.基金项目
吉林省科委国际合作项目资助，批准号20100735，项目名称《新型神经假体的研制及在脊髓损伤后膀胱功能重建中的应用》@@@@International Cooperation Projects in Jilin Province Science and Technology Commission, No.20100735 （）
