中国组织工程研究Issue(37)：6676-6681,6.DOI:10.3969/j.issn.2095-4344.2013.37.022
激素性股骨头坏死模型：不同构建技术分析
Model of steroid-induced avascular necrosis of the femoral head:Analysis of different construction techniques
李瑞琦 1张国平 1李宜炯 1李亚丽 1任立中 1张宇宸 1王伟 1高宏阳 1吕亚军1
作者信息
- 1. 河北医科大学第一医院骨科,河北省石家庄市,050031
- 折叠
摘要
Abstract
BACKGROUND:To construct a normal animal model of femoral head necrosis contributes to the research of the pathogenesis of femoral head necrosis, which can provide theoretical basis for the prevention and treatment of femoral head necrosis. <br> OBJECTIVE:To research the experimental effect of lipopolysaccharide combined with dexamethasone injection in the induction of rabbit femoral head necrosis. <br> METHODS:Thirty-six New Zealand white rabbits were randomly divided into model group (n=21) and control group (n=15). The rabbits in the model group were injected with 10μg/kg lipopolysaccharide daily and continuous for 2 days, and then injected with 25 mg/kg dexamethasone daily for 3 days continuously. The rabbits in the control group were injected with the normal saline at the same volume. <br> RESULTS AND CONCLUSION:After 4 weeks, the X-ray film of the rabbit in the model group showed the joint gaps were widened, the density was increased, the articular subchondral bone mineral density was increased, the femoral head was flat, trabecular bone was fuzzy, the boundaries between subchondral bone and cancel ous bone was unclear, and the patchy high-density areas were observed in the femoral head with shortened femoral neck. The bone mineral density of partial femoral head was measured with dual-energy X-ray absorptiometry, and found that the bone mineral density of femoral head and the bone mineral content of the model group were significantly lower than those of the control group (P<0.05). Histological section observation showed that the bone cel lacuna was empty and shal ow, fat cel s were increased and vascular thrombosis was observed, meanwhile, the osteonecrosis rate and lacunae rate of the survival animals were significantly higher than those in the control group. Dexamethasone combined with lipopolysaccharide can effectively construct the model of steroid-induced avascular necrosis of the femoral head.关键词
组织构建/组织构建学术探讨/动物模型/激素性股骨头坏死/致死原因/内毒素脂多糖/地塞米松/骨密度/骨坏死/病理学分类
医药卫生引用本文复制引用
李瑞琦,张国平,李宜炯,李亚丽,任立中,张宇宸,王伟,高宏阳,吕亚军..激素性股骨头坏死模型：不同构建技术分析[J].中国组织工程研究,2013,(37):6676-6681,6.
