中国组织工程研究Issue(38)：6753-6758,6.DOI:10.3969/j.issn.2095-4344.2013.38.008
D，L-聚乳酸基形状记忆聚合物的生物安全性和细胞相容性
Shape-memory polymer based on D,L-poly(lactic acid):Biosecurity and cytocompatibility
摘要
Abstract
BACKGROUND:Previous experiments have prepared shape memory polymer based on D,L-poly(lactic acid). According to domestic technical requirements for biological y evaluating biomaterials and medical equipments, tissue-engineered grafts must be subjected to preclinical experiment for biosecurity and cytocompatibility evaluation. <br> OBJECTIVE:To observe the biosecurity of the shape memory polymer based on D,L-poly(lactic acid). <br> METHODS:(1) Bacterial endotoxin test:polymer extract, endotoxin working standard solution and checking <br> standard water were added into limulus reagent, respectively. (2) Sensitization test:Polymer extract+Freund’s complete adjuvant+physiological saline and Freund’s complete adjuvant+physiological saline were injected into the scapula of Kunming mice. After induction by intradermal injection, local induction and excitation, stimulate skin erythema and edema degree were observed in animals. (3) Acute toxicity test:Kunming mice received intraperitoneal injection of 100%, 50%, 25%polymer leaching solution and physiological saline, respectively. (4) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay for cel proliferation:The direct method was that human umbilical cord vascular endothelial cel s were inoculated onto the polymer film, lactic acid and glass, respectively;the indirect method was that human umbilical cord vascular endothelial cel s were inoculated into the polymer leaching solution, acrylamide solution and 1640 culture solution. <br> RESULTS AND CONCLUSION:This shape memory polymer based on D,L-poly(lactic acid) is free of bacterial contamination in compliance with the biosecurity standards, and it has no al ergenic and toxicity but has good cytocompatibility.关键词
生物材料/材料生物相容性/D,L-聚乳酸形状记忆聚合物/生物安全性/细胞相容性/省级基金分类
医药卫生引用本文复制引用
须建,彭坤,甘哓玲,王易振,段巧玲,李永刚..D，L-聚乳酸基形状记忆聚合物的生物安全性和细胞相容性[J].中国组织工程研究,2013,(38):6753-6758,6.基金项目
重庆市教委项目(KJ112501)，重庆市卫生局项目(2012-2-257)，重庆医药高等专科学校重点科研项目(2012103)@@@@the Project of Chongqing Educational Committee, No. KJ112501 （KJ112501）
Project of Chongqing Health Bureau, No.2012-2-257 （）
the Major Scientific Research Project of Chongqing Medical and Pharmaceutical Col ege, No.2012103 （）
