中国组织工程研究Issue(38)：6779-6784,6.DOI:10.3969/j.issn.2095-4344.2013.38.012
细胞共培养技术促进类前微血管结构发生
Cell co-culture technology accelerates premicrovascular-like structure formation
摘要
Abstract
BACKGROUND:Constructing a three-dimensional tissue-like structure in vitro plays a critical role in modern tissue engineering and regenerative medicine. Several advances have been made in the past decade. However, it is stil a chal enge to promote microvascular-like structure formation and improve limited nutritional transportation, thereby promoting cel viability. <br> OBJECTIVE:To explore the feasibility of constructing a three-dimensional microvascular-like structure through the co-culture technique. <br> METHODS:Human bone marrow mesenchymal stem cel s and human endothelial cel s were co-cultured on a three-dimensional porous silk scaffold. Cel proliferation was analyzed by Pico-green DNA assay. Their growth profiles were evaluated by scanning electron microscope and laser scanning confocal microscopy, respectively. The mRNA levels of von Wil ebrand factor and CD31, two key functional markers of endothelial cel s, in the co-cultured endothelial cel s was assayed by real-time quantitative reverse transcription-PCR. <br> RESULTS AND CONCLUSION:The three-dimensional culture system constructed by the silk scaffold and bone marrow mesenchymal stem cel s provided an ideal microenvironment for cel growth and proliferation in vitro. Moreover, this microenvironment was capable of promoting endothelial cel differentiation evidenced by their significantly improved mRNA levels of von Wil ebrand factor and CD31. Premicrovascular-like structure was also observed in the co-cultures under the confocal microscope. Thus, al the data supported that the unique co-culture system could promote endothelial cel differentiation and self-assembling in vitro. This culture system provides a robust tool for the studies addressing microvessel-based tissue engineering.关键词
生物材料/生物材料基础实验/三维培养/血管内皮细胞/支架/CD31/vWF/微环境/国家自然科学基金分类
医药卫生引用本文复制引用
王纪文,李向东,魏国峰..细胞共培养技术促进类前微血管结构发生[J].中国组织工程研究,2013,(38):6779-6784,6.基金项目
国家自然科学基金面上项目资助(81173125)@@@@the General Program of National Natural Science Foundation of China, No.81173125 （81173125）
