原子能科学技术Issue(10)：1685-1691,7.DOI:10.7538/yzk.2013.47.10.1685
人促甲状腺激素酶促化学发光免疫分析方法的建立
Establishment of Chemiluminescence Immunassay for Thyroid Stimulating Hormone
李丽波 1刘一兵 1官国英 1刘中瑞 1侯惠仁 1梁瑞鹏1
作者信息
- 1. 原子高科股份有限公司,北京,102413
- 折叠
摘要
Abstract
The chemiluminescence immunoassay for thyroid stimulating hormone in human serum was developed . Two monoclonal antibodies of TSH were used in the assay ,one of which was labeled with horseradish peroxidase (HRP) and the other was coated on the microtiter plate .Luminol was used as the substrate of HRP and hydrogen peroxide was introduced into this system . The standard range of the method is 0.1-100 mIU/L . The assay sensitivity is 0.04 mIU/L . The intra- and inter-assay coeffi-cients of variance are 3.98%-6.48% and 4.61%-13.1% .Analytical recovery is 95.8%-117.4% .The correlation coefficient between measured and expected values is more than 0.99 .The cross reaction rate with LH is less than 1.62% ,and that with FSH and HCG is less than 0.05% and 0.02% ,respectively .Compared with determine value clinically in chemiluminescence immunoassay (CLIA ) kit from Roche company ,the correlative equation is y = 1.10 x -0.207 , and correlation coefficient is 0.969 . Compared with immunoradiometric assay (IRMA ) kit ,the correlative equation is y= 1.00 x+ 0.191 , and correlation coefficient is 0.965 . This method is rapid and convenient , and it is suitable for clinical and research application .关键词
促甲状腺激素/辣根过氧化物酶/酶促化学发光免疫分析Key words
thyroid stimulating hormone/horseradish peroxidase/chemiluminescence immunoassay分类
医药卫生引用本文复制引用
李丽波,刘一兵,官国英,刘中瑞,侯惠仁,梁瑞鹏..人促甲状腺激素酶促化学发光免疫分析方法的建立[J].原子能科学技术,2013,(10):1685-1691,7.
