中国动物检疫Issue(8)：70-74,5.
赤羽病SYBR Green I实时荧光PCR方法的建立和应用
Development and Application of SYBR Green I Real-time PCR Assay for Detecting Akabane virus
摘要
Abstract
In the study, the primers were designed and synthesized according to the conservative S se-quence of AKAV. The genes were amplified by reverse transcription polymerase chain reaction(RT-PCR) and cloned to the PGM-T vector. The reaction parameters were optimized to develop SYBR Green I fluores-cence quantitative PCR assay. It was shown that the fluorescence quantitative PCR assay could detect 926 copies/25μL of plasmid DNA and its specificity and reproducibility were very good. The method was very useful for laboratories working with early and rapid identification of AKAV for cattle importatim and expor-tation.关键词
AKAV/实时荧光定量PCR/SYBR Green IKey words
AKAV/Real-time PCR/SYBR Green I分类
农业科技引用本文复制引用
陈圣军,孔繁德,徐淑菲,张吉红,唐泰山..赤羽病SYBR Green I实时荧光PCR方法的建立和应用[J].中国动物检疫,2013,(8):70-74,5.基金项目
福建省自然科学基金科技计划项目（2010IK016）；厦门市科技计划项目（3502Z2010012）；宁波市科技计划项目（2007C10057）和国家质检总局科技计划项目 （）
