中国动物检疫Issue(8)：78-82,5.
贝类中副溶血弧菌和沙门氏菌荧光定量PCR快速检测方法的建立
Rapid detection of Vibrio parahaemolyticus and Salmonella sp. in shell ifshes by Real-time quantitative PCR
摘要
Abstract
Rapid detection of pathogens is essential for rapid import inspection of live aquatic animals. In the paper, a real-time quantitative PCR assay was developed for rapid detection of V. parahaemolyticus and Salmonella sp. in shellfishes. PCR primers were designed targeting the species-specific gene gyrB of V. parahaemolyticus and the fimY gene of Salmonella sp. The specificity and repeatability of the method were also tested. The results showed that both V. parahaemolyticus strains and Salmonella sp. exhibited typical curves while the other negative control strains were not amplified, indicating the specificity of the assay. The standard curves showed good linearity at the range between 1.53×105 and 1.53×109 copies/μL for V. parahaemolyticus, and between 4.89×106 and 4.89×1010 copies/μL for Salmonella sp. The rapidness and reliability of the assay should be enough for the quick inspection of live imported shellfishes.关键词
副溶血弧菌/沙门氏菌/荧光定量PCR/贝类Key words
Vibrio parahaemolyticus/Salmonella sp./Real-time PCR/shellfishes分类
医药卫生引用本文复制引用
倪梦丽,杨冰,王春德,陈颖,孙善华..贝类中副溶血弧菌和沙门氏菌荧光定量PCR快速检测方法的建立[J].中国动物检疫,2013,(8):78-82,5.基金项目
国家质量检验检疫总局科技计划项目 （）
