中国肺癌杂志2015，Vol.18Issue(4)：212-218,7.DOI:10.3779/j.issn.1009-3419.2015.04.01
免疫组化法检测NSCLC患者EGFR突变的相关研究
Immunohistochemical detections ofEGFRstatus in NSCLC
王杰 1刘畅 1钟殿胜 1徐东波 2宁超 1马晴1
作者信息
- 1. 天津医科大学总医院肿瘤科, 天津,300052
- 2. 天津医科大学病理学教研室, 天津,300070
- 折叠
摘要
Abstract
Background and objective Patients with non-small cell lung cancer (NSCLC) harboring mutations of the epidermal growth factor receptor (EGFR) respond well to EGFR-tyrosine kinase inhibitor therapy. Immunohistochemistry (IHC) is a simple and widely used technique in clinical pathology laboratories. IHC also features cost effectiveness and rapid detection ofEGFR mutations compared with molecular methods. hTis study aims to determine the accuracy of IHC forEGFR mutation detection in NSCLC.Methods Specimens (obtained from surgery or biopsy) from 97 NSCLC cases were stained through IHC with mutation-specific antibodies. The clinicopathological features of patients with positive immunostaining results were analyzed. Positive specimens were subjected to liquid chip technology to detect the actual EGFR status. Forty NSCLC specimens obtained from surgery and conifrmed to haveEGFR mutations through liquid chip technology were collect-ed. These specimens were then subjected to IHC analyses with mutation-speciifc antibodies. The sensitivity of IHC in detect-ingEGFR mutations was calculated.Results Seventeen of the 97 NSCLC specimens were stained positive, and positive results were mostly observed in females, patients with adenocarcinoma, and non-smokers. About 76.9% of specimens with positive IHC results harbored mutations. The sensitivity of IHC was 40% among the 40 cases identiifed as containingEGFR mutations through liquid chip technology.Conclusion The strong positive immunostaining result is accurate, but the sensitivity of the method may not be optimal and signiifcantly varies in different studies. The widespread application of IHC in clinics must be further investigated.关键词
肺肿瘤/表皮生长因子受体/突变/免疫组化法/液相芯片法Key words
Lung neoplasms/Epidermal growth factor receptor/Mutation/Immunohistochemistry/Liquid chip technology引用本文复制引用
王杰,刘畅,钟殿胜,徐东波,宁超,马晴..免疫组化法检测NSCLC患者EGFR突变的相关研究[J].中国肺癌杂志,2015,18(4):212-218,7.
