中国水产科学Issue(5)：931-938,8.DOI:10.3724/SP.J.1118.2013.00931
基于线粒体COI基因序列的5种鲤养殖品种遗传多样性研究
Study of DNA barcoding and genetic variation based on the mito-chondrial COI gene sequences in five carp varieties
摘要
Abstract
The mitochondrial COI gene of five cyprinoid aquaculture varieties were sequenced and their genetic diversity and cluster analysis were compared. Eight haplotypes were detected from 100 individuals, of which 94.06%belonged to five haplotypes (H1, H2, H4, H5, H6). Cyprinus carpio blue var has only one haplotype, C. c. Red mirror carp has two haplotypes, and the other varieties including Cyprinus carpio Frigid carp, C. c. Songpu carp and C. c. Songpu mirror carp had five. Haplotype diversity (Hd) was 0.160±0.070~0.811±0.055, the highest Hd frequency was observed in Frigid carp. The AMOVA and cluster analysis revealed that genetic distance be-tween varieties (50.28%) was a little higher than that within varieties (49.72%), and the FST ranged from 0.711 4?0.831 2. Compared with the others varieties, Cyprinus carpio blue var exhibited the biggest differences. Ac-cording to the evolutionary tree based on genetic distance, five varieties could be divided into three groups, the first group included Frigid carp and Red mirror carp, the second included Songpu mirror carp and Songpu carp, and the last included Cyprinus carpio blue var. With the exception of Cyprinus carpio blue var, all varieties shared the same haplotypes (H2, H4, H5, H6), which may be due to cross breeding. This study also indicated that DNA barcoding is a practical tool for analyzing the hereditary of different varieties. However, some limitations still ex-ist.关键词
鲤养殖品种/遗传多样性/DNA条形码/COI基因Key words
cyprinoid aquaculture breeds/genetic diversity/DNA barcode/COI gene分类
农业科技引用本文复制引用
单云晶,鲁翠云,李超,张明昭,顾颖,孙效文..基于线粒体COI基因序列的5种鲤养殖品种遗传多样性研究[J].中国水产科学,2013,(5):931-938,8.基金项目
农业部公益性行业科研专项(200903045) （200903045）
国家863计划项目(2011AA100402-5) （2011AA100402-5）
国家科技基础条件平台建设运行项目 （）
