中国水产科学Issue(5)：1066-1075,10.DOI:10.3724/SP.J.1118.2013.01066
不同C：N条件下一株溶藻弧菌对不同种类氮源的吸收特性
Nitrogen source assimilation of a Vibrio alginolyticus strain under different C/N ratios
摘要
Abstract
Nitrogen assimilation characteristics of a Vibrio alginolyticus strain (Z5) were investigated under both single (organic nitrogen, NH4-N, or NO2-N) and mixed nitrogen sources (organic nitrogen and NH4-N, organic nitrogen and NH4-N, or NH4-N, and NO2-N) at C/N ratios of 5, 10, 15, and 20 respectively. Activity of protease, glutamine synthetase, and nitrite reductase, which are all involved in nitrogen metabolism, was also determined. The results showed that both organic and inorganic nitrogen assimilation rates of V. alginolyticus were affected by C/N ratio. NH4-N and NO2-N assimilation rates under a C/N ratio of 20 were 3.39 and 2.25 times higher, respec-tively, than those under a C/N ratio of 5. There were no significant differences in the assimilation of NH4-N and NO2-N between C/N ratios of 15 and 20 (P>0.05). The NH4-N and NO2-N assimilation processes may be NH4-N→bacterial protein and NO2-N→NH4-N→bacterial protein, respectively. Inorganic nitrogen (NH4-N and NO2-N) assimilation rates increased with higher C/N; the protein nitrogen content of the bacteria enriched from NH4-N and NO2-N increased from 25.0% and 19.4% under a C/N of 5 to 41.3% and 43.0% under a C/N of 20, respectively. In addition, glutamine synthetase activity was significantly regulated by NH4-N while protease and NiR activity were not regulated significantly by the nitrogen species.关键词
溶藻弧菌/氮源/吸收/酶调控/C:NKey words
Vibrio alginolyticus/nitrogen source/assimilation/enzyme regulating/C:N ratio分类
农业科技引用本文复制引用
单洪伟,高磊,马甡,魏大鹏,张家松..不同C：N条件下一株溶藻弧菌对不同种类氮源的吸收特性[J].中国水产科学,2013,(5):1066-1075,10.基金项目
国家科技支撑计划课题(2011BAD13B10) （2011BAD13B10）
农业部公益性行业科研专项经费资助项目(201103034) （201103034）
