中国烟草科学Issue(4)：55-59,5.DOI:10.3969/j.issn.1007-5119.2013.04.012
山东烟草白绢病病原鉴定及室内防治药剂筛选
Identification and Pharmaceutical Screening on Plate of Sclerotium rolfsii Sacc. Causing Southern Blight on Tobacco
摘要
Abstract
The morphology, biological characteristics, 18SrDNA and fungicide screening of the pathogen isolated from the root of tobacco from Feixian in Shandong province were studied. The results showed that pathogen of FBJ had pathogenicity of tobacco. Through morphological characteristics and 18S rDNA sequence analysis, FBJ was Sclerotium rolfsii Sacc. It could cause southern blight on tobacco. Biological characteristics displayed that the pathogen of FBJ grew well at the pH range of 5.0-10.0, with the optimal pH of 9.0. Hypha and sclerotia viability were completely lost when hyphal agar plugs and sclerotia incubated at 50℃ 10 minutes. Fungicide testing on PDA plates revealed that 70%Mancozeb showed the greatest inhibition of hyphal growth and sclerotia germination of S. rolfsii.关键词
烟草/白绢病/鉴定/毒力测定Key words
tobacco/Sclerotium rolfsii Sacc./identification/toxicity determination分类
农业科技引用本文复制引用
王静,赵杰,钱玉梅,王秀芳,孔凡玉,张成省,冯超..山东烟草白绢病病原鉴定及室内防治药剂筛选[J].中国烟草科学,2013,(4):55-59,5.基金项目
中国烟草总公司重点项目“全国烟草有害生物调查研究”（110200902065）；山东省烟草专卖局项目“山东省烟草有害生物调查研究”（201001）” （）
