浙江医学Issue(16)：1490-1493,4.
雷公藤内酯醇对小鼠肾小球足细胞裂孔隔膜核心蛋白表达干预作用的研究
摘要
Abstract
Objective To investigate the effect of triptolide (TP) on the expression of GPSD core proteins in mouse podocytes induced by high glucose. Methods The cultured mouse podocyte were randomly divided into control group1, control group2, high glucose group and TP treatment groups, which were further divided into low, middle and high dose subgroups. Podocyte exposed to 25 mmol/L glucose were treated with different doses of triptolide (8,16 and 32ng/ml). The expressions of nephrin, podocin and CD2AP mRNA were examined by RT- PCR. The expressions of nephrin and podocin protein were detected by indirect immunoflorescence technique (IIFT). Results Compared to control group, the expression of nephrin, podocin and CD2AP mRNA in high glucose group were significantly decreased (P<0.05 or P<0.01). Compared to high glucose group, the expression of nephrin, podocin and CD2AP mRNA were significantly up- regulated (P<0.05) in 3 TP- treatment groups, and the expression was highest in middle dose group (P<0.05). IIFT demonstrated that the inhibited expression of nephrin and podocin by high- glucose was significantly up- regulated in the middle dose group. Conclusion The expressions of nephrin, podocin and CD2AP in the mouse podocyte are inhibited in high glucose condition. These core proteins inhibited in high glucose can be sig-nificantly up- regulated by triptolide, indicating that it may attenuate the proteinuria in diabetic hephropathy.关键词
雷公藤内酯醇/糖尿病肾病/肾小球足细胞裂孔隔膜/核心蛋白Key words
Triptolide/DN/GPSD/Core protein引用本文复制引用
叶迅,洪郁芝,徐新鹏..雷公藤内酯醇对小鼠肾小球足细胞裂孔隔膜核心蛋白表达干预作用的研究[J].浙江医学,2013,(16):1490-1493,4.基金项目
浙江省中医药管理局科研基金资助项目 （）
