安徽农业科学2016，Vol.44Issue(14)：97-100,4.
超声辅助酶法回收南极磷虾壳中蛋白质的研究
Research on the Protein Extracted from Antarctic Krill Shell with Ultrasonic-assisted Enzymatic Method
摘要
Abstract
In order to maximally recover the protein from Antarctic krill shell, the extraction technology of enzymatic hydrolysis method with Ultrasonic-assisted was studied.The influence of the different proteinases, ratio of solid-to-liquid, pH value, enzymolysis time and tempera-ture , ratio of enzyme to substrate and ultrasonic power on the recovery of protein was tested.With orthogonal experiment, the optimal process parameters were formed, which was as follows:the pH value was in natural condition; the ratio of solid to liquid, 1∶10 g /mL; the ultrasonic power, 150 W; the ratio of enzyme to substrate, 5 000 U /g protein; the temperature, 50 ℃; and the treating time, 2.5 h at 50 ℃.The ex-traction efficiency of protein in the optimized conditions was 87.42%.Alkaline protease had the strongest capability in hydrolyzing protein.As a result, the protein in Antarctic krill shell could be recovered with the technique to the most extent and the kinds of resource could be fully u-tilized.关键词
酶解/超声波/南极磷虾下脚料/蛋白质Key words
Enzymatic hydrolysis/Ultrasonic/Antarctic krill shell/Protein分类
农业科技引用本文复制引用
徐洋,吕大强,王舒婷,曹雪,于波,姜启兴,许艳顺,于沛沛,夏文水..超声辅助酶法回收南极磷虾壳中蛋白质的研究[J].安徽农业科学,2016,44(14):97-100,4.基金项目
“十二五”国家“863”计划项目（2011AA090801）；农业科技成果转化资金项目（2014GB2C100312）。 （）
